In silico cloning and chromosomal localization of EST sequences that are related to leaf senescence using nulli-tetrasomes in wheat
In silico cloning and chromosomal localization of EST sequences that are related to leaf senescence using nulli-tetrasomes in wheat
复制标题
使用小麦无效四体对与叶片衰老相关的 EST 序列进行计算机克隆和染色体定位
DOI：
10.1556/0806.43.2015.014
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发表时间：
2015-06
影响因子：
1.6
通讯作者：
Luo PG
中科院分区：
文献类型：
--
作者：
Li LQ;Liu TG;Ren ZL;Luo PG
Leaf senescence is a notably important trait that limits the yield and biomass accumulation of agronomic crops. Therefore, determining the chromosomal position of the expression sequence tags (ESTs) that are associated with leaf senescence is notably interesting in the manipulation of leaf senescence for crop improvement. A total of 32 ESTs that were previously identified during the delaying leaf senescence stage in the stay-green wheat cultivar CN17 were mapped to 42 chromosomes, a chloroplast, a mitochondrion, and a ribosome using in silico mapping. Then, we developed 19 pairs of primers based on these sequences and used them to determine the polymorphisms between the stay-green cultivars (CN12, CN17, and CN18) and the control cultivar MY11. Among the 19 pairs of primers, 5 pairs produced polymorphisms between the stay-green cultivar and the non-stay-green control. Further studies of Chinese Spring nullisomic-tetrasomics show that JK738991 is mapped to 3B, JK738983 is mapped to 5D, and JK738989 is mapped to 2A, 4A, and 3D. The other two ESTs, JK738994 and JK739003, were not assigned to a chromosome using the Chinese Spring nullisomic-tetrasomics, which indicates that these ESTs may be derived from rye DNA in the wide cross. In particular, the ESTs that produce polymorphisms are notably useful in identifying the stay-green cultivar using molecular marker-assisted selection. The results also suggest that the in silico mapping data, even from a comparison genomic analysis based on the homogeneous comparison, are useful at some points, but the data were not always reliable, which requires further investigation using experimental methods.
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影响因子：
--
作者：
Luo Peigao;Ren Zhenglong;Feng Juan
通讯作者：
Feng Juan
影响因子：
6.9
作者：
Thomas, H;Howarth, CJ
通讯作者：
Howarth, CJ
影响因子：
7.3
作者：
Luo, P. G.;Deng, K. J.;Ren, Z. L.
通讯作者：
Ren, Z. L.
DOI：
--
发表时间：
2003
期刊：
Journal of Sichuan Agricultural University
影响因子：
--
作者：
R. Zheng
通讯作者：
R. Zheng
小麦-黑麦双倍体揭示的串联重复序列、调控元件和启动子区域的变异。
影响因子：
3.1
作者：
Tang, Zong-Xiang;Fu, Shu-Lan;Zhang, Huai-Qiong
通讯作者：
Zhang, Huai-Qiong