The Effects of Irreversible Electroporation on the Achilles Tendon: An Experimental Study in a Rabbit Model.
The Effects of Irreversible Electroporation on the Achilles Tendon: An Experimental Study in a Rabbit Model.
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不可逆电穿孔对跟腱的影响：兔模型的实验研究
DOI：
10.1371/journal.pone.0131404
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Li Z
中科院分区：
文献类型：
--
作者：
Song Y;Zheng J;Yan M;Ding W;Xu K;Fan Q;Li Z
目的评价不可逆电穿孔消融对兔跟腱的潜在影响，并比较电穿孔消融和射频消融后标本的组织病理学和生物力学变化。方法选用6月龄雄性新西兰大白兔140只。将动物随机分为两组，射频消融组70只，电穿孔组70只。使用典型的电穿孔（1800 V/cm，90个脉冲）和射频消融（功率控制模式）方案将原位消融直接应用于兔跟腱。进行组织学和生物力学评价，以检查电穿孔消融和射频消融随时间的影响。结果电穿孔和射频消融均能在靶区产生完全的细胞消融。射频消融后3天，热损伤导致肌腱断裂。相比之下，电穿孔消融的跟腱保留了它们的生物力学特性，并且在该时间点未显示出可检测到的断裂。电穿孔消融的肌腱在2周内表现出恢复的迹象，包括成腱细胞再生和血管生成，其整体结构的恢复在12周内是明显的。结论当应用电穿孔消融实体瘤时，主要优点可能是由于电穿孔消融靶向细胞膜的独特能力，对邻近肌腱或韧带的附带损伤最小化。这一优势可能对重要肌腱或韧带附近的肿瘤消融领域产生重大影响。
Background To evaluate the potential effects of irreversible electroporation ablation on the Achilles tendon in a rabbit model and to compare the histopathological and biomechanical changes between specimens following electroporation ablation and radiofrequency ablation. Methods A total of 140 six-month-old male New Zealand rabbits were used. The animals were randomly divided into two groups, 70 in the radiofrequency ablation group and 70 in the electroporation group. In situ ablations were applied directly to the Achilles tendons of rabbits using typical electroporation (1800 V/cm, 90 pulses) and radiofrequency ablation (power control mode) protocols. Histopathological and biomechanical evaluations were performed to examine the effects of electroporation ablation and radiofrequency ablation over time. Results Both electroporation and radiofrequency ablation produced complete cell ablation in the target region. Thermal damage resulted in tendon rupture 3 days post radiofrequency ablation. In contrast, electroporation-ablated Achilles tendons preserved their biomechanical properties and showed no detectable rupture at this time point. The electroporation-ablated tendons exhibited signs of recovery, including tenoblast regeneration and angiogenesis within 2 weeks, and the restoration of their integral structure was evident within 12 weeks. Conclusions When applying electroporation to ablate solid tumors, major advantage could be that collateral damage to adjacent tendons or ligaments is minimized due to the unique ability of electroporation ablation to target the cell membrane. This advantage could have a significant impact on the field of tumor ablation near vital tendons or ligaments.
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DOI：
10.1102/1470-7330.2009.9028
发表时间：
2009-10-02
期刊：
Cancer imaging : the official publication of the International Cancer Imaging Society
影响因子：
--
作者：
Gillams A
通讯作者：
Gillams A
影响因子：
3.8
作者：
Dustmann, M.;Schmidt, T.;Scheffler, S. U.
通讯作者：
Scheffler, S. U.
影响因子：
1.8
作者：
Ilhami, K;Eray, BM;Levent, A
通讯作者：
Levent, A
影响因子：
2.8
作者：
Miller, L;Leor, J;Rubinsky, B
通讯作者：
Rubinsky, B
影响因子：
64.8
作者：
COHEN, RE;HOOLEY, CJ;MCCRUM, NG
通讯作者：
MCCRUM, NG