A disposable microcapsule array chip fabricated by ice printing combined with isothermal amplification for Salmonella DNA detection.
A disposable microcapsule array chip fabricated by ice printing combined with isothermal amplification for Salmonella DNA detection.
复制标题
冰印结合等温扩增一次性微胶囊阵列芯片用于沙门氏菌DNA检测
DOI：
10.1039/c8ra07045h
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发表时间：
2018-11-23
期刊：
影响因子：
3.9
通讯作者：
Li, Zhihong
中科院分区：
文献类型：
--
作者：
He, Enqi;Cao, Ting;Cai, Liangyuan;Guo, Dan;Zhou, Yinglin;Zhang, Xinxiang;Li, Zhihong
本研究将冰印技术与DNA等温扩增技术相结合，构建了一种新型的沙门氏菌DNA检测微胶囊阵列芯片。反应溶液通过冰印刷预先密封在微胶囊阵列芯片中。为了保护相对脆弱的DNA等温扩增体系，引入了一层额外的聚苯乙烯（PS）膜来隔离反应液与光聚合物前体，这是为DNA扩增反应提供清洁、稳定环境的关键步骤。检测操作可以通过简单的注射器将样品DNA注射到微胶囊中来完成，并且可以在90分钟内通过颜色变化直接获得结果。该方法具有良好的灵敏度、特异性和稳定性。
In this work, a novel microcapsule array chip was fabricated for the detection of Salmonella DNA by integrating an ice-printing technique with DNA isothermal amplification. Reaction solutions were previously sealed in the microcapsule array chip via ice printing. To protect the relatively fragile DNA isothermal amplification system, an extra polystyrene (PS) film was introduced to isolate the reaction solution from photopolymer precursor, which was proved to be a vital step for providing a clean and stable environment for DNA amplification reaction. Detection operation can be done by simply injecting sample DNA into the microcapsule by an easily accessible syringe, and the result can be directly obtained through color change within 90 minutes. This method shows good sensitivity, specificity and stability.
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影响因子：
12.6
作者：
Fang, Zhiyuan;Wu, Wei;Zeng, Lingwen
通讯作者：
Zeng, Lingwen
影响因子：
6
作者：
Li, Junjie;Zhai, Ligong;Zhao, Haizhen
通讯作者：
Zhao, Haizhen
影响因子：
--
作者：
Mori, Y;Kitao, M;Notomi, T
通讯作者：
Notomi, T
影响因子：
3.9
作者：
Thom NK;Lewis GG;Yeung K;Phillips ST
通讯作者：
Phillips ST
影响因子：
2.7
作者：
Gnoth, C.;Johnson, S.
通讯作者：
Johnson, S.