Identification of four plasma microRNAs as potential biomarkers in the diagnosis of male lung squamous cell carcinoma patients in China.
Identification of four plasma microRNAs as potential biomarkers in the diagnosis of male lung squamous cell carcinoma patients in China.
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鉴定四种血浆 microRNA 作为诊断中国男性肺鳞状细胞癌患者的潜在生物标志物
DOI：
10.1002/cam4.1490
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发表时间：
2018-06
期刊：
影响因子：
4
通讯作者：
Liu P
中科院分区：
文献类型：
--
作者：
Shan X;Zhang H;Zhang L;Zhou X;Wang T;Zhang J;Shu Y;Zhu W;Wen W;Liu P
肺鳞状细胞癌患者血浆中表达异常的microRNAs（miRNAs）可作为肺鳞状细胞癌诊断的生物标志物。在初始筛选阶段使用定量逆转录-聚合酶链反应（qRT-PCR）基于包括3份男性LSCC合并样本和1份正常对照（NC）合并样本（每10份样本合并为1份合并样本）的Quantiqon样本组进行miRNA表达。在通过qRT-PCR的训练（32个LSCC vs. 31个NC）、测试（55个LSCC vs. 55个NC）和外部验证（15个LSCC vs. 15个NC）阶段之后，鉴定了用于LSCC检测的四种miRNA特征（miR-181 a-5 p、miR-21 - 5 p、miR-106 a-5 p和miR-93 - 5 p）。在训练、测试和外部验证阶段，四种miRNA组的受试者工作特征（ROC）曲线下面积（AUC）分别为0.795、0.827和0.914。然后，在LSCC组织样本中探索了这四种miRNAs（23例LSCC vs. 23例NC），它们的表达显著上调。然而，在血浆外泌体中发现的四种miRNA中没有一种显著上调，除了具有边界显著性的miR-93 - 5 p（16例LSCC vs. 16例NC）。综上所述，我们的研究建立了一个4-miRNA外周血签名，这在一定程度上有助于诊断中国男性LSCC患者。
Dysregulated microRNAs (miRNAs) in the plasma of patients with lung squamous cell carcinoma (LSCC) might serve as biomarkers for LSCC diagnosis. The expression of miRNAs was performed using quantitative reverse transcription–polymerase chain reaction (qRT‐PCR) on the basis of Exiqon panels in the initial screening phase including three male LSCC pool samples and one normal control (NC) pool sample (per 10 samples were pooled as one pool sample). After the training (32 LSCC vs. 31 NCs), the testing (55 LSCC vs. 55 NCs), and the external validation (15 LSCC vs. 15 NCs) stages via qRT‐PCR, a four‐miRNA signature (miR‐181a‐5p, miR‐21‐5p, miR‐106a‐5p, and miR‐93‐5p) was identified for LSCC detection. Areas under the receiver operating characteristic (ROC) curve (AUC) of the four‐miRNA panel for the training, the testing, and the external validation phases were 0.795, 0.827, and 0.914, respectively. Then, the four miRNAs were explored in LSCC tissue samples (23 LSCC vs. 23 NCs), and their expression was significantly up‐regulated. However, none of the four miRNAs found significantly up‐regulated in plasma exosomes expect miR‐93‐5p with borderline significance (16 LSCC vs. 16 NCs). In summary, our study established a four‐miRNA peripheral plasma signature, which contributed to diagnosing male LSCC patients in China to a certain degree.
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DOI：
10.3322/caac.21244
发表时间：
2014-09
期刊：
CA: a cancer journal for clinicians
影响因子：
--
作者：
Berindan-Neagoe I;Monroig Pdel C;Pasculli B;Calin GA
通讯作者：
Calin GA
影响因子：
6.4
作者：
Duell EJ;Lujan-Barroso L;Sala N;Deitz McElyea S;Overvad K;Tjonneland A;Olsen A;Weiderpass E;Busund LT;Moi L;Muller D;Vineis P;Aune D;Matullo G;Naccarati A;Panico S;Tagliabue G;Tumino R;Palli D;Kaaks R;Katzke VA;Boeing H;Bueno-de-Mesquita HBA;Peeters PH;Trichopoulou A;Lagiou P;Kotanidou A;Travis RC;Wareham N;Khaw KT;Ramon Quiros J;Rodríguez-Barranco M;Dorronsoro M;Chirlaque MD;Ardanaz E;Severi G;Boutron-Ruault MC;Rebours V;Brennan P;Gunter M;Scelo G;Cote G;Sherman S;Korc M
通讯作者：
Korc M
影响因子：
--
作者：
Chen, Gang;Shen, Zhi-Li;Zhou, Rong-Ping
通讯作者：
Zhou, Rong-Ping
影响因子：
4.3
作者：
Fang, Ling;Du, William W.;Yang, Burton B.
通讯作者：
Yang, Burton B.
影响因子：
5.4
作者：
Guo, Li-Juan;Zhang, Qing-Yun
通讯作者：
Zhang, Qing-Yun