Expression of Protein Acetylation Regulators During Peripheral Nerve Development, Injury, and Regeneration.
Expression of Protein Acetylation Regulators During Peripheral Nerve Development, Injury, and Regeneration.
复制标题
DOI：
10.3389/fnmol.2022.888523
复制
发表时间：
2022
影响因子：
4.8
通讯作者：
Ding, Fei
中科院分区：
文献类型：
--
作者：
Sun, Junjie;Ji, Yuhua;Liang, Qingyun;Ming, Mengru;Chen, Yuhan;Zhang, Qi;Zhou, Songlin;Shen, Mi;Ding, Fei
蛋白质乙酰化是一种重要的翻译后修饰，受乙酰基转移酶和去乙酰基酶的调控，参与周围神经的许多生理和病理变化。目前对坐骨神经发育、损伤和再生过程中蛋白乙酰化调节因子的表达变化尚缺乏系统的研究。在这里，我们测序和分析小鼠坐骨神经发育过程中和损伤后的转录组。我们发现，大多数调控因子的表达变化遵循“发育与再生一致，与损伤相反”的规律。用全乙酰化抗体进行的免疫印迹也揭示了发育和再生是乙酰化增加的过程，而损伤是乙酰化减少的过程。此外，我们使用生物信息学方法分析了两个关键调控因子组蛋白去乙酰化酶1（Hdac1）和赖氨酸乙酰转移酶2b（Kat2b）的可能下游分子，发现它们与许多调控细胞周期的基因相关。我们的研究结果从蛋白质乙酰化的角度提供了对坐骨神经发育、损伤和再生之间关系的深入了解。
Protein acetylation, regulated by acetyltransferases and deacetylases, is an important post-translational modification that is involved in numerous physiological and pathological changes in peripheral nerves. There is still no systematical analysis on the expression changes of protein acetylation regulators during sciatic nerve development, injury, and regeneration. Here, we sequenced and analyzed the transcriptome of mouse sciatic nerves during development and after injury. We found that the changes in the expression of most regulators followed the rule that “development is consistent with regeneration and opposite to injury.” Immunoblotting with pan-acetylated antibodies also revealed that development and regeneration are a process of increased acetylation, while injury is a process of decreased acetylation. Moreover, we used bioinformatics methods to analyze the possible downstream molecules of two key regulators, histone deacetylase 1 (Hdac1) and lysine acetyltransferase 2b (Kat2b), and found that they were associated with many genes that regulate the cell cycle. Our findings provide an insight into the association of sciatic nerve development, injury, and regeneration from the perspective of protein acetylation.
登录
查看更多内容
影响因子：
56.9
作者：
Choudhary, Chunaram;Kumar, Chanchal;Mann, Matthias
通讯作者：
Mann, Matthias
DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
64.8
作者：
Lu Y;Brommer B;Tian X;Krishnan A;Meer M;Wang C;Vera DL;Zeng Q;Yu D;Bonkowski MS;Yang JH;Zhou S;Hoffmann EM;Karg MM;Schultz MB;Kane AE;Davidsohn N;Korobkina E;Chwalek K;Rajman LA;Church GM;Hochedlinger K;Gladyshev VN;Horvath S;Levine ME;Gregory-Ksander MS;Ksander BR;He Z;Sinclair DA
通讯作者：
Sinclair DA
影响因子：
6.1
作者：
Liu JH;Tang Q;Liu XX;Qi J;Zeng RX;Zhu ZW;He B;Xu YB
通讯作者：
Xu YB
影响因子：
64.8
作者：
Deardorff, Matthew A.;Bando, Masashige;Nakato, Ryuichiro;Watrin, Erwan;Itoh, Takehiko;Minamino, Masashi;Saitoh, Katsuya;Komata, Makiko;Katou, Yuki;Clark, Dinah;Cole, Kathryn E.;De Baere, Elfride;Decroos, Christophe;Di Donato, Nataliya;Ernst, Sarah;Francey, Lauren J.;Gyftodimou, Yolanda;Hirashima, Kyotaro;Hullings, Melanie;Ishikawa, Yuuichi;Jaulin, Christian;Kaur, Maninder;Kiyono, Tohru;Lombardi, Patrick M.;Magnaghi-Jaulin, Laura;Mortier, Geert R.;Nozaki, Naohito;Petersen, Michael B.;Seimiya, Hiroyuki;Siu, Victoria M.;Suzuki, Yutaka;Takagaki, Kentaro;Wilde, Jonathan J.;Willems, Patrick J.;Prigent, Claude;Gillessen-Kaesbach, Gabriele;Christianson, David W.;Kaiser, Frank J.;Jackson, Laird G.;Hirota, Toru;Krantz, Ian D.;Shirahige, Katsuhiko
通讯作者：
Shirahige, Katsuhiko