The conjugation of SUMO to the transcription factor MYC2 functions in blue light-mediated seedling development in Arabidopsis.
The conjugation of SUMO to the transcription factor MYC2 functions in blue light-mediated seedling development in Arabidopsis.
复制标题
DOI：
10.1093/plcell/koac142
复制
发表时间：
2022-07-30
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
光感受器信号传导的一个关键功能是协调调节大量基因以优化植物的生长和发育。基本螺旋环螺旋（bHLH）转录因子MYC2在蓝光下拟南芥发育过程中调控基因表达至关重要。在这里，我们证明了蓝光诱导MYC2的sumo化。非sumoylatable MYC2在抑制蓝光介导的光形态发生方面不如野生型MYC2有效。MYC2与SUMO蛋白酶SPF1 （SUMO PROTEASE RELATED TO fertility）和SPF2相互作用。蓝光照射可促进SPF1和SPF2的降解，增强MYC2的SUMOylation。表型分析显示SPF1/SPF2作为蓝光介导的光形态发生的正调节因子具有冗余功能。我们的数据表明，SUMO偶联不影响MYC转录因子的二聚化，但调节MYC2与其同源DNA顺式元件以及与泛素连接酶Plant U-box 10 （PUB10）的相互作用。最后，我们发现不可SUMO修饰的MYC2比WT更不稳定，与PUB10的相互作用更强。综上所述，我们得出结论，SUMO可以作为MYC2泛素介导降解的对位物，从而增强其在蓝光信号传导中的功能。SUMO通过调节bHLH转录因子MYC2的DNA结合能力和稳定性，调控蓝光介导的拟南芥幼苗发育。
A key function of photoreceptor signaling is the coordinated regulation of a large number of genes to optimize plant growth and development. The basic helix loop helix (bHLH) transcription factor MYC2 is crucial for regulating gene expression in Arabidopsis thaliana during development in blue light. Here we demonstrate that blue light induces the SUMOylation of MYC2. Non-SUMOylatable MYC2 is less effective in suppressing blue light-mediated photomorphogenesis than wild-type (WT) MYC2. MYC2 interacts physically with the SUMO proteases SUMO PROTEASE RELATED TO FERTILITY1 (SPF1) and SPF2. Blue light exposure promotes the degradation of SPF1 and SPF2 and enhances the SUMOylation of MYC2. Phenotypic analysis revealed that SPF1/SPF2 function redundantly as positive regulators of blue light-mediated photomorphogenesis. Our data demonstrate that SUMO conjugation does not affect the dimerization of MYC transcription factors but modulates the interaction of MYC2 with its cognate DNA cis-element and with the ubiquitin ligase Plant U-box 10 (PUB10). Finally, we show that non-SUMOylatable MYC2 is less stable and interacts more strongly with PUB10 than the WT. Taken together, we conclude that SUMO functions as a counterpoint to the ubiquitin-mediated degradation of MYC2, thereby enhancing its function in blue light signaling. SUMO regulates blue light-mediated Arabidopsis seedling development by regulating the DNA binding ability and stability of the bHLH transcription factor MYC2.
登录
查看更多内容
影响因子：
4.5
作者：
Hammoudi V;Fokkens L;Beerens B;Vlachakis G;Chatterjee S;Arroyo-Mateos M;Wackers PFK;Jonker MJ;van den Burg HA
通讯作者：
van den Burg HA
影响因子：
11.6
作者：
Anderson, JP;Badruzsaufari, E;Kazan, K
通讯作者：
Kazan, K
影响因子：
6.9
作者：
Garrido E;Srivastava AK;Sadanandom A
通讯作者：
Sadanandom A
影响因子：
5.6
作者：
Mazur MJ;van den Burg HA
通讯作者：
van den Burg HA
影响因子：
11.6
作者：
Catala, Rafael;Ouyang, Jian;Chua, Nam-Hai
通讯作者：
Chua, Nam-Hai