The cell morphogenesis ANGUSTIFOLIA (AN) gene, a plant homolog of CtBP/BARS, is involved in abiotic and biotic stress response in higher plants.
The cell morphogenesis ANGUSTIFOLIA (AN) gene, a plant homolog of CtBP/BARS, is involved in abiotic and biotic stress response in higher plants.
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DOI：
10.1186/1471-2229-13-79
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发表时间：
2013-05-14
影响因子：
5.3
通讯作者：
Kotchoni SO
中科院分区：
文献类型：
--
作者：
Gachomo EW;Jimenez-Lopez JC;Smith SR;Cooksey AB;Oghoghomeh OM;Johnson N;Baba-Moussa L;Kotchoni SO
ANGUSTIFOLIA（AN）是CtBP家族蛋白之一，在微管依赖性细胞形态发生中起主要作用。哺乳动物AN同源物的微阵列分析表明，AN可能作为一个转录激活因子和调节因子的广泛的基因。AN突变体的遗传特征表明，AN可能参与细胞形态调控以外的多种生物学过程。利用反向遗传学的方法，我们在本文中提供的遗传，生化和生理证据狭叶植物的作用，在其他新的生物功能，如非生物和生物胁迫反应在高等植物。T-DNA敲除an-t1突变体不仅表现出先前描述的沙枣无效突变体的所有表型，而且在脱水和病原菌攻击下比野生型更好地应对。胁迫耐受伴随着细胞内H2 O2含量的稳态调节、细胞膜脂过氧化产生的丙二醛（MDA）以及胁迫应答基因的过表达。我们的研究结果表明，狭叶功能超出细胞形态控制，通过直接或间接的功能蛋白质相互作用网络介导的其他生物过程，如干旱和病原体的攻击。我们的研究结果表明，ANGUSTIFOLIA基因参与控制细胞形态发生，非生物和生物胁迫反应在高等植物的几个生化途径。我们的研究结果表明，在植物狭叶的体内功能一直被忽视，它需要进一步研究微管依赖的细胞形态发生以外。
ANGUSTIFOLIA (AN), one of the CtBP family proteins, plays a major role in microtubule-dependent cell morphogenesis. Microarray analysis of mammalian AN homologs suggests that AN might function as a transcriptional activator and regulator of a wide range of genes. Genetic characterization of AN mutants suggests that AN might be involved in multiple biological processes beyond cell morphology regulation. Using a reverse genetic approach, we provide in this paper the genetic, biochemical, and physiological evidence for ANGUSTIFOLIA’s role in other new biological functions such as abiotic and biotic stress response in higher plants. The T-DNA knockout an-t1 mutant exhibits not only all the phenotypes of previously described angustifolia null mutants, but also copes better than wild type under dehydration and pathogen attack. The stress tolerance is accompanied by a steady-state modulation of cellular H2O2 content, malondialdehyde (MDA) derived from cellular lipid peroxidation, and over-expression of stress responsive genes. Our results indicate that ANGUSTIFOLIA functions beyond cell morphology control through direct or indirect functional protein interaction networks mediating other biological processes such as drought and pathogen attacks. Our results indicate that the ANGUSTIFOLIA gene participates in several biochemical pathways controlling cell morphogenesis, abiotic, and biotic stress responses in higher plants. Our results suggest that the in vivo function of plant ANGUSTIFOLIA has been overlooked and it needs to be further studied beyond microtubule-dependent cell morphogenesis.
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影响因子：
7.3
作者：
Kotchoni, Simeon O.;Kuhns, Christine;Bartels, Dorothea
通讯作者：
Bartels, Dorothea
DOI：
10.1073/pnas.92.23.10467
发表时间：
1995-11-07
影响因子：
11.1
作者：
SCHAEPER, U;BOYD, JM;CHINADURAI, G
通讯作者：
CHINADURAI, G
影响因子：
64.5
作者：
HULSKAMP, M;MISERA, S;JURGENS, G
通讯作者：
JURGENS, G
影响因子：
11.4
作者：
Nibu, Y;Zhang, HL;Levine, M
通讯作者：
Levine, M
影响因子：
9.2
作者：
Zhang, Chunhua;Kotchoni, Simeon O.;Szymanski, Daniel B.
通讯作者：
Szymanski, Daniel B.