Molecular analysis of phosphomannomutase (PMM) genes reveals a unique PMM duplication event in diverse Triticeae species and the main PMM isozymes in bread wheat tissues.
Molecular analysis of phosphomannomutase (PMM) genes reveals a unique PMM duplication event in diverse Triticeae species and the main PMM isozymes in bread wheat tissues.
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磷酸甘露糖变位酶 (PMM) 基因的分子分析揭示了多种小麦科物种中独特的 PMM 复制事件以及面包小麦组织中的主要 PMM 同工酶。
DOI：
10.1186/1471-2229-10-214
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发表时间：
2010-10-05
影响因子：
5.3
通讯作者：
Wang D
中科院分区：
文献类型：
--
作者：
Yu C;Li Y;Li B;Liu X;Hao L;Chen J;Qian W;Li S;Wang G;Bai S;Ye H;Qin H;Shen Q;Chen L;Zhang A;Wang D
磷酸甘露糖变位酶(PMM)是真核生物中的一种重要酶。然而，对PMM基因及其在作物中的功能还知之甚少。在这里，我们报道了面包小麦及其近缘种的PMM基因的分子进化和生化分析。在面包小麦中发现了两组同源的PMM基因(TaPMM-1和TaPMM-2)，在面包小麦的二倍体祖先和其他许多二倍体小麦种中发现了两个对应的PMM基因。产生PMM-1和PMM-2的复制事件发生在二倍体小麦基因组辐射之前。小麦及其近缘种的PMM基因家族在纯化选择下可能发生较大的进化。在6个TaPMM基因中，PMM-1成员的转录水平较高，其重组蛋白均具有酶活性。然而，PMM-2同源基因表现出较低的转录水平，其中两个也是不活跃的。TaPMM-A1、B1和D1可能是面包小麦组织中的主要活性同工酶。这三种同工酶不同于大麦和青冈的同工酶，这是因为它们对高温的耐受性更强。我们的工作鉴定了编码面包小麦及其近缘种PMM同工酶的基因，发现了在不同小麦族物种中独特的PMM复制事件，并揭示了面包小麦组织中主要的PMM同工酶活性。本研究结果加深了对真核生物PMM进化的理解，有助于进一步研究PMM在面包小麦温度适应性中的作用。
Phosphomannomutase (PMM) is an essential enzyme in eukaryotes. However, little is known about PMM gene and function in crop plants. Here, we report molecular evolutionary and biochemical analysis of PMM genes in bread wheat and related Triticeae species. Two sets of homoeologous PMM genes (TaPMM-1 and 2) were found in bread wheat, and two corresponding PMM genes were identified in the diploid progenitors of bread wheat and many other diploid Triticeae species. The duplication event yielding PMM-1 and 2 occurred before the radiation of diploid Triticeae genomes. The PMM gene family in wheat and relatives may evolve largely under purifying selection. Among the six TaPMM genes, the transcript levels of PMM-1 members were comparatively high and their recombinant proteins were all enzymatically active. However, PMM-2 homoeologs exhibited lower transcript levels, two of which were also inactive. TaPMM-A1, B1 and D1 were probably the main active isozymes in bread wheat tissues. The three isozymes differed from their counterparts in barley and Brachypodium distachyon in being more tolerant to elevated test temperatures. Our work identified the genes encoding PMM isozymes in bread wheat and relatives, uncovered a unique PMM duplication event in diverse Triticeae species, and revealed the main active PMM isozymes in bread wheat tissues. The knowledge obtained here improves the understanding of PMM evolution in eukaryotic organisms, and may facilitate further investigations of PMM function in the temperature adaptability of bread wheat.
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影响因子：
10.7
作者：
Ding, KY;Zhou, K;Shen, Y
通讯作者：
Shen, Y
影响因子：
3.3
作者：
Cho, SH;Garvin, DF;Muehlbauer, GJ
通讯作者：
Muehlbauer, GJ
影响因子：
3.5
作者：
Heykants, L;Schollen, E;Matthijs, G
通讯作者：
Matthijs, G
影响因子：
10.7
作者：
Badr, A;Müller, K;Salamini, F
通讯作者：
Salamini, F
DOI：
10.1073/pnas.072223799
发表时间：
2002-06-11
影响因子：
11.1
作者：
Huang, S;Sirikhachornkit, A;Gornicki, P
通讯作者：
Gornicki, P