Oxidation-Induced Increase In Photoreactivity of Bovine Retinal Lipid Extract.
Oxidation-Induced Increase In Photoreactivity of Bovine Retinal Lipid Extract.
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DOI：
10.1007/s12013-017-0832-3
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发表时间：
2017-12
影响因子：
2.6
通讯作者：
Pawlak A
中科院分区：
文献类型：
--
作者：
Koscielniak A;Serafin M;Duda M;Oles T;Zadlo A;Broniec A;Berdeaux O;Gregoire S;Bretillon L;Sarna T;Pawlak A
哺乳动物视网膜含有高水平的多不饱和脂肪酸，包括二十二碳六烯酸（22：6）（DHA），其对氧化高度敏感。研究表明，DHA氧化的产物之一--原位生成的羧乙基吡咯（CEP）引起视网膜蛋白质的修饰，并诱导外层视网膜的炎症反应。这些促成因素可能在年龄相关性黄斑变性（AMD）的发展中发挥作用。也有可能一些脂质氧化产物是光反应性的，并且在用蓝光照射时可能产生活性氧物质。因此，在这项工作中，我们分析了从牛神经视网膜中提取的脂质的光反应性的氧化诱导的变化。牛神经视网膜的脂质组成与人视网膜的脂质组成非常相似，这使得牛组织成为研究氧化的人视网膜脂质的光反应性和潜在光毒性的方便模型。采用气相色谱（GC）和液相色谱-电喷雾离子源-质谱（LC-ESI-MS）联用技术对牛神经视网膜Folch提取物（BRex）的脂质组成进行了分析。由BRex制备的脂质体，用空气平衡，在黑暗中在37 ° C下氧化长达400小时。用EPR-氧饱和法和EPR-自旋捕获法研究了BRex在不同氧化阶段的光反应性。利用时间分辨的特征磷光检测技术，在1270 nm处测量了BRex的单线态氧（1O2，1 Δ g）的光生过程。为了确定脂质组分对分析的牛视网膜Folch提取物的光反应性的贡献，还研究了类似于BRex的重量百分比（w/w %）比率的所选合成脂质的混合物。尽管存在强大的内源性抗氧化剂，如α-生育酚和玉米黄质，但牛神经视网膜的Folch提取物对氧化非常敏感。非氧化和氧化BRex光生单线态氧具有中等的量子产率。蓝光诱导牛神经视网膜Folch提取液产生超氧阴离子强烈依赖于氧化时间。观察到的光反应性的研究提取物逐渐增加，在其体外氧化。
The mammalian retina contains a high level of polyunsaturated fatty acids, including docosahexaenoic acid (22:6) (DHA), which are highly susceptible to oxidation. It has been shown that one of the products of DHA oxidation—carboxyethylpyrrole (CEP), generated in situ, causes modifications of retinal proteins and induces inflammation response in the outer retina. These contributing factors may play a role in the development of age-related macular degeneration (AMD). It is also possible that some of the lipid oxidation products are photoreactive, and upon irradiation with blue light may generate reactive oxygen species. Therefore, in this work we analysed oxidation-induced changes in photoreactivity of lipids extracted from bovine neural retinas. Lipid composition of bovine neural retinas closely resembles that of human retinas making the bovine tissue a convenient model for studying the photoreactivity and potential phototoxicity of oxidized human retinal lipids. Lipid composition of bovine neural retinas Folch’ extracts (BRex) was determined by gas chromatography (GC) and liquid chromatography coupled to an electrospray ionization source-mass spectrometer (LC-ESI-MS) analysis. Liposomes prepared from BRex, equilibrated with air, were oxidized in the dark at 37 °C for up to 400 h. The photoreactivity of BRex at different stages of oxidation was studied by EPR-oximetry and EPR-spin trapping. Photogeneration of singlet oxygen (1O2, 1Δg) by BRex was measured using time-resolved detection of the characteristic phosphorescence at 1270 nm. To establish contribution of lipid components to the analysed photoreactivity of Folch’ extract of bovine retinas, a mixture of selected synthetic lipids in percent by weight (w/w %) ratio resembling that of the BRex has been also studied. Folch’s extraction of bovine neural retinas was very susceptible to oxidation despite the presence of powerful endogenous antioxidants such as α-tocopherol and zeaxanthin. Non-oxidized and oxidized BRex photogenerated singlet oxygen with moderate quantum yield. Blue-light induced generation of superoxide anion by Folch’ extract of bovine neural retinas strongly depended on the oxidation time. The observed photoreactivity of the studied extract gradually increased during its in vitro oxidation.
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影响因子：
3.9
作者：
CHAMULITRAT, W;HUGHES, MF;MASON, RP
通讯作者：
MASON, RP
DOI：
10.1016/1011-1344(91)80266-k
发表时间：
1991-10-01
影响因子：
5.4
作者：
CONN, PF;SCHALCH, W;TRUSCOTT, TG
通讯作者：
TRUSCOTT, TG
DOI：
10.1016/s0378-4347(98)00431-9
发表时间：
1998-12-11
期刊：
JOURNAL OF CHROMATOGRAPHY B
影响因子：
--
作者：
Dachtler, M;Kohler, K;Albert, K
通讯作者：
Albert, K
影响因子：
3.4
作者：
Acar, Niyazi;Gregoire, Stephane;Bretillon, Lionel
通讯作者：
Bretillon, Lionel
影响因子：
1.8
作者：
Bernstein, Paul S.;Delori, Francois C.;Richer, Stuart;van Kuijk, Frederik J. M.;Wenzel, Adam J.
通讯作者：
Wenzel, Adam J.