A Realtime and Continuous Assessment of Cortisol in ISF Using Electrochemical Impedance Spectroscopy.
A Realtime and Continuous Assessment of Cortisol in ISF Using Electrochemical Impedance Spectroscopy.
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DOI：
10.1016/j.sna.2011.04.028
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发表时间：
2011-12-01
影响因子：
4.6
通讯作者：
Bhansali, Shekhar
中科院分区：
文献类型：
--
作者：
Venugopal, Manju;Arya, Sunil K.;Chornokur, Ganna;Bhansali, Shekhar
关键词：
本研究描述了一种新型传感器的功能，以测量皮质醇浓度的间质液（ISF）的人类受试者。ISF是通过真空压力从皮肤角质层上产生的微孔中提取的。毛孔是通过将近红外激光聚焦在附着在皮肤上的一层黑色染料材料上而产生的。皮肤穿孔后，毛孔可存活约三天。皮质醇测量基于电化学阻抗（EIS）技术。用二硫代双（琥珀酰亚胺基丙酸酯）自组装单分子膜（SAM）功能化的金微电极阵列已被用来制作一个超灵敏的，一次性的，电化学皮质醇免疫传感器。该生物传感器已成功用于ISF中皮质醇的体外测定。在实验室设置中的测试表明，传感器对皮质醇浓度在1 pm至100 nM范围内表现出线性响应。一项小型试点临床研究表明，与使用酶联免疫测定（ELISA）方法进行的商业评估相比，体外免疫传感器读数与唾液和ISF中的皮质醇水平相关性良好。此外，可以在受试者的ISF和在24小时时间段内收集的唾液样品之间建立昼夜节律。ISF中的皮质醇水平被发现可靠地高于唾液。本研究建立了使用基于阻抗的生物传感器架构的一次性，可穿戴皮质醇检测器的可行性。预计的商业体内实时皮质醇传感器设备，除了微创外，还将允许连续ISF采集和皮质醇监测超过24小时，即使受试者处于睡眠状态。即将到来的是，这种传感器可以连接到无线健康监测系统，该系统可以通过现有的广域网（如互联网和蜂窝电话网络）传输传感器数据，以实现对受试者的实时远程监测。
This study describes the functioning of a novel sensor to measure cortisol concentration in the interstitial fluid (ISF) of a human subject. ISF is extracted by means of vacuum pressure from micropores created on the stratum corneum layer of the skin. The pores are produced by focusing a near infrared laser on a layer of black dye material attached to the skin. The pores are viable for approximately three days after skin poration. Cortisol measurements are based on electrochemical impedance (EIS) technique. Gold microelectrode arrays functionalized with Dithiobis (succinimidyl propionate) self-assembled monolayer (SAM) have been used to fabricate an ultrasensitive, disposable, electrochemical cortisol immunosensor. The biosensor was successfully used for in-vitro measurement of cortisol in ISF. Tests in a laboratory setup show that the sensor exhibits a linear response to cortisol concentrations in the range 1 pm to 100 nM. A small pilot clinical study showed that in-vitro immunosensor readings, when compared with commercial evaluation using enzyme-linked immunoassay (ELISA) method, correlated well with cortisol levels in saliva and ISF. Further, circadian rhythm could be established between the subject's ISF and the saliva samples collected over 24 hours time-period. Cortisol levels in ISF were found reliably higher than in saliva. This Research establishes the feasibility of using impedance based biosensor architecture for a disposable, wearable cortisol detector. The projected commercial in-vivo real-time cortisol sensor device, besides being minimally invasive, will allow continuous ISF harvesting and cortisol monitoring over 24 hours even when the subject is asleep. Forthcoming, this sensor could be interfaced to a wireless health monitoring system that could transfer sensor data over existing wide-area networks such as the internet and a cellular phone network to enable real-time remote monitoring of subjects.
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影响因子：
16.2
作者：
STERNBERG, F;MEYERHOFF, C;PFEIFFER, EF
通讯作者：
PFEIFFER, EF
DOI：
10.1111/j.1365-2362.2005.01552.x
发表时间：
2005-10-01
影响因子：
5.5
作者：
Abdallah, BM;Beck-Nielsen, H;Gaster, M
通讯作者：
Gaster, M
影响因子：
5.4
作者：
Stout, Philip J;Racchini, Joel R;Hilgers, Michael E
通讯作者：
Hilgers, Michael E
DOI：
10.1016/s0022-2143(97)90044-5
发表时间：
1997-10-01
期刊：
JOURNAL OF LABORATORY AND CLINICAL MEDICINE
影响因子：
--
作者：
Bantle, JP;Thomas, W
通讯作者：
Thomas, W
DOI：
10.1016/0022-4731(75)90068-0
发表时间：
1975-01-01
影响因子：
4.1
作者：
KRIEGER, DT
通讯作者：
KRIEGER, DT