Monitoring changes of docosahexaenoic acid-containing lipids during the recovery process of traumatic brain injury in rat using mass spectrometry imaging.
Monitoring changes of docosahexaenoic acid-containing lipids during the recovery process of traumatic brain injury in rat using mass spectrometry imaging.
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质谱成像监测大鼠脑外伤恢复过程中含二十二碳六烯酸脂质的变化
DOI：
10.1038/s41598-017-05446-2
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发表时间：
2017-07-11
影响因子：
4.6
通讯作者：
Li Z
中科院分区：
文献类型：
--
作者：
Guo S;Zhou D;Zhang M;Li T;Liu Y;Xu Y;Chen T;Li Z
Brain lipid homoeostasis is critical during recovery process after traumatic brain injury (TBI). In this study, we integrated liquid extraction and electrosonic spray ionization technology to develop an ionization device coupled with a Fourier transform ion cyclotron resonance mass spectrometer for imaging of docosahexaenoic acid (DHA)-containing lipids on rat brain tissues. The ion images of the brain tissue sections from the normal rats and the rats after TBI at acute phase (0 and 1 day) and chronic phase (3, 5, and 7 days) were obtained. The imaging results indicate that the levels of DHA and lyso-phosphatidylethanolamine (22:6) in the injury area of TBI rats increased significantly at the acute phase and subsequently decreased at the chronic phase. But the levels of DHA-containing phospholipids including phosphatidylethanolamine (PE)(P-18:0/22:6), PE(18:0/22:6), and phosphatidylserine (18:0/22:6) decreased at the acute phase and gradually increased at the chronic phase in the injury area accompanied by the morphogenesis and wound healing. These findings indicate that the DHA may participate in the recovery process of brain injury. This is the first report toin situdetect the changes in the levels of DHA and DHA-containing lipids in the TBI model.
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影响因子：
7.4
作者：
Laskin, Julia;Heath, Brandi S.;Roach, Patrick J.;Cazares, Lisa;Semmes, O. John
通讯作者：
Semmes, O. John
DOI：
10.1007/s13361-011-0122-z
发表时间：
2011-06
影响因子：
3.2
作者：
Hankin JA;Farias SE;Barkley RM;Heidenreich K;Frey LC;Hamazaki K;Kim HY;Murphy RC
通讯作者：
Murphy RC
影响因子：
3.3
作者：
Koizumi, S.;Yamamoto, S.;Namba, H.
通讯作者：
Namba, H.
影响因子：
4.1
作者：
Mills, James D.;Bailes, Julian E.;Sears, Barry
通讯作者：
Sears, Barry
影响因子：
4.9
作者：
Nefliu, M;Venter, A;Cooks, RG
通讯作者：
Cooks, RG