Effects of lactation and pregnancy on gene expression of endometrium of Holstein cows at day 17 of the estrous cycle or pregnancy.
Effects of lactation and pregnancy on gene expression of endometrium of Holstein cows at day 17 of the estrous cycle or pregnancy.
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泌乳和妊娠对荷斯坦母牛子宫内膜基因表达的影响在发情周期或妊娠的第17天。
DOI：
10.3168/jds.2011-5114
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发表时间：
2012-10
影响因子：
3.5
通讯作者：
Thatcher WW
中科院分区：
文献类型：
--
作者：
Cerri RL;Thompson IM;Kim IH;Ealy AD;Hansen PJ;Staples CR;Li JL;Santos JE;Thatcher WW
目的探讨哺乳和妊娠对动情周期和妊娠期第17天子宫内膜基因表达的影响。33头母牛分娩后随机分为哺乳期组（L, n = 17）和非哺乳期组（NL, n = 16）。对奶牛进行定时人工授精（TAI）的排卵同步程序；L区10头奶牛和NL区12头奶牛进行了人工授精。在相当于TAI的第17天屠宰，收集环间子宫内膜组织。通过DNA芯片分析，测定了妊娠奶牛（L, n = 8； NL, n = 6）和未授精周期奶牛（L, n = 7； NL, n = 4）的基因表达。选择差异表达基因，p值<0.01，绝对表达量bb0 40。此外，以bbb1.5倍效应作为妊娠影响基因的标准。共有210个基因受哺乳影响差异调节（136个下调，74个上调），702个基因受妊娠影响差异调节（407个下调，295个上调）。孕乳互作效应影响61个基因。奶牛体内基因的上调和下调与防御反应和干扰素调节因子、细胞粘附和细胞外基质等基因本体相关。通过对泌乳奶牛上调和下调基因的基因本体分析，揭示了与免疫球蛋白样折叠、免疫应答、COMM结构域和非膜结合细胞器相关的术语。哺乳时上调的一些基因，如IGHG1、IGLL1、IGK和TRD，与免疫功能有关，特别是对B细胞和γδ T细胞。与肢体、神经发育和葡萄糖稳态相关的发育基因（如DKK1、RELN、PDK4）在哺乳期被下调，而RELN也被检测到相互作用。上述与免疫功能相关的基因和受泌乳状态影响的子宫内膜中表达的发育基因可能是干预提高泌乳奶牛生育能力的候选基因。
Objectives were to determine effects of lactation and pregnancy on endometrial gene expression on d 17 of the estrous cycle and pregnancy. Heifers (n = 33) were assigned randomly after parturition to lactating (L, n = 17) or nonlactating (NL, n = 16) groups. Cows were subjected to an ovulation synchronization program for a timed artificial insemination (TAI); 10 cows in L and 12 in NL were inseminated. Slaughter occurred 17 d after the day equivalent to TAI, and intercaruncular endometrial tissues were collected. Gene expression was determined by DNA microarray analysis for pregnant (L, n = 8; NL, n = 6) and noninseminated cyclic (L, n = 7; NL, n = 4) cows. Differentially expressed genes were selected with a P-value <0.01 and absolute expression >40. In addition, a fold effect >1.5 was used as a criterion for genes affected by pregnancy. In total, 210 genes were differentially regulated by lactation (136 downregulated and 74 upregulated), and 702 genes were differentially regulated by pregnancy (407 downregulated and 295 upregulated). The interaction effect of pregnancy and lactation affected 61 genes. Genes up- and downregulated in pregnant cows were associated with several gene ontology terms, such as defense response and interferon regulatory factor, cell adhesion, and extracellular matrix. The gene ontology analyses of up- and downregulated genes of lactating cows revealed terms related to immunoglobulin-like fold, immune response, COMM domain, and non-membrane-bounded organelle. Several genes upregulated by lactation, such as IGHG1, IGLL1, IGK, and TRD, were related to immune function, particularly for B cells and γδ T cells. Developmental genes related to limb and neural development and glucose homeostasis (e.g., DKK1, RELN, PDK4) were downregulated by lactation, whereas an interaction was also detected for RELN. The stated genes associated with immune function and developmental genes expressed in the endometrium affected by lactational state are possible candidate genes for interventions to improve fertility of lactating dairy cows.
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影响因子：
64.8
作者：
Glinka, A;Wu, W;Niehrs, C
通讯作者：
Niehrs, C
影响因子：
4.6
作者：
Loor, Juan J.;Everts, Robin E.;Lewin, Harris A.
通讯作者：
Lewin, Harris A.
影响因子：
3.8
作者：
Cooke, Flavia N. T.;Pennington, Kathleen A.;Ealy, Alan D.
通讯作者：
Ealy, Alan D.
影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
11.4
作者：
Grotewold, L;Rüther, U
通讯作者：
Rüther, U