Limited sex-biased neural gene expression patterns across strains in Zebrafish (Danio rerio).
Limited sex-biased neural gene expression patterns across strains in Zebrafish (Danio rerio).
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DOI：
10.1186/1471-2164-15-905
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发表时间：
2014-10-17
期刊：
影响因子：
4.4
通讯作者：
Godwin J
中科院分区：
文献类型：
--
作者：
Wong RY;McLeod MM;Godwin J
雄性和雌性脊椎动物通常在一系列特征上存在差异，从形态到生理再到行为，这些特征受到社会环境、体内激素和遗传环境等因素的影响。然而，脊椎动物大脑中基因表达谱的性别差异才刚刚开始被理解。鱼类为哺乳动物和鸟类的性别差异研究提供了一个独特的补充，因为鱼类在发育甚至成年期间表现出性别二态特征和行为的极端可塑性和不稳定性。因此，硬骨鱼模型可以为性别分化提供额外的见解。这项研究的目的是确定大脑性别差异的神经转录机制。在这项研究中，我们通过rna测序检测了四种斑马鱼的全脑性别偏倚基因表达。随后，我们通过使用加权基因共表达网络分析检查性别之间的基因网络动态，进行了系统级分析。令人惊讶的是，只有61个基因（约占分析基因的0.4%）在所有四种菌株中显示出显著的性别效应，其中48个差异是雄性偏向的。其中一些基因与类固醇激素的生物合成有关。尽管在压力相关行为的表现上存在性别差异，但基础转录水平并不能预测行为表现的强度。WGCNA发现只有一个模块与性别显著相关。有趣的是，比较两性之间的模块间动态，发现只有适度的保存。进一步，我们确定了性别特异性基因模块。尽管斑马鱼在形态、生理和行为上存在差异，但在斑马鱼中存在有限的性别偏倚神经基因表达。此外，性别偏向的基因与激素生物合成有关，这表明性类固醇激素可能是硬骨鱼性行为可塑性的关键因素。一种可能的机制是通过调节特定的大脑基因网络。本文的在线版本（doi:10.1186/1471-2164-15-905）包含补充材料，可供授权用户使用。
Male and female vertebrates typically differ in a range of characteristics, from morphology to physiology to behavior, which are influenced by factors such as the social environment and the internal hormonal and genetic milieu. However, sex differences in gene expression profiles in the brains of vertebrates are only beginning to be understood. Fishes provide a unique complement to studies of sex differences in mammals and birds given that fish show extreme plasticity and lability of sexually dimorphic characters and behaviors during development and even adulthood. Hence, teleost models can give additional insight into sexual differentiation. The goal of this study is to identify neurotranscriptomic mechanisms for sex differences in the brain. In this study we examined whole-brain sex-biased gene expression through RNA-sequencing across four strains of zebrafish. We subsequently conducted systems level analyses by examining gene network dynamics between the sexes using weighted gene coexpression network analysis. Surprisingly, only 61 genes (approximately 0.4% of genes analyzed) showed a significant sex effect across all four strains, and 48 of these differences were male-biased. Several of these genes are associated with steroid hormone biosynthesis. Despite sex differences in a display of stress-related behaviors, basal transcript levels did not predict the intensity of the behavioral display. WGCNA revealed only one module that was significantly associated with sex. Intriguingly, comparing intermodule dynamics between the sexes revealed only moderate preservation. Further we identify sex-specific gene modules. Despite differences in morphology, physiology, and behavior, there is limited sex-biased neural gene expression in zebrafish. Further, genes found to be sex-biased are associated with hormone biosynthesis, suggesting that sex steroid hormones may be key contributors to sexual behavioral plasticity seen in teleosts. A possible mechanism is through regulating specific brain gene networks. The online version of this article (doi:10.1186/1471-2164-15-905) contains supplementary material, which is available to authorized users.
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WGCNA：用于加权相关网络分析的 R 包。
影响因子：
3
作者：
Langfelder P;Horvath S
通讯作者：
Horvath S
影响因子：
4.3
作者：
Langfelder P;Luo R;Oldham MC;Horvath S
通讯作者：
Horvath S
影响因子：
4.4
作者：
Catalán A;Hutter S;Parsch J
通讯作者：
Parsch J
DOI：
10.1007/978-3-7091-0932-8_7
发表时间：
2012-01-01
期刊：
SYNAPTIC PLASTICITY: DYNAMICS, DEVELOPMENT AND DISEASE
影响因子：
--
作者：
Frischknecht, Renato;Gundelfinger, Eckart D.
通讯作者：
Gundelfinger, Eckart D.
影响因子：
10.7
作者：
Griffin, Robert M.;Dean, Rebecca;Friberg, Urban
通讯作者：
Friberg, Urban