Population and sex differences in Drosophila melanogaster brain gene expression.
Population and sex differences in Drosophila melanogaster brain gene expression.
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DOI：
10.1186/1471-2164-13-654
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发表时间：
2012-11-21
期刊：
影响因子：
4.4
通讯作者：
Parsch J
中科院分区：
文献类型：
--
作者：
Catalán A;Hutter S;Parsch J
基因调控的变化被认为是生物体适应环境的关键。转录组分析可用于鉴定生态适应的候选基因，但可能因组织、性别或个体之间基因表达的差异而复杂化。在这里，我们使用高通量RNA测序的一个单一的果蝇组织检测大脑特异性差异的基因表达的性别之间和两个群体之间，一个从祖先的物种范围在撒哈拉以南非洲和一个从最近殖民的物种范围在欧洲。相对较少的基因（<100个）在大脑中显示出性别二态性表达，但在X染色体上存在性别偏好基因，特别是男性偏好基因的富集。来自非洲和欧洲种群的果蝇之间有超过340个基因在大脑表达上存在差异，种群间差异在雄性和雌性之间高度相关。差异表达的基因包括那些参与应激反应，嗅觉和解毒。表达差异与转座因子插入在两个涉及杀虫剂抗性的基因（Cyp6g1和CHKov1）。对大脑转录组的分析揭示了许多基因在种群之间的表达差异，这些基因在以前使用整只苍蝇的研究中没有检测到。几乎没有证据表明大脑中存在性别特异性调节适应，因为在男性和女性中观察到群体间的大多数表达差异。X染色体上性二态表达基因的富集与影响体细胞组织中性别偏向表达的剂量补偿机制一致。
Changes in gene regulation are thought to be crucial for the adaptation of organisms to their environment. Transcriptome analyses can be used to identify candidate genes for ecological adaptation, but can be complicated by variation in gene expression between tissues, sexes, or individuals. Here we use high-throughput RNA sequencing of a single Drosophila melanogaster tissue to detect brain-specific differences in gene expression between the sexes and between two populations, one from the ancestral species range in sub-Saharan Africa and one from the recently colonized species range in Europe. Relatively few genes (<100) displayed sexually dimorphic expression in the brain, but there was an enrichment of sex-biased genes, especially male-biased genes, on the X chromosome. Over 340 genes differed in brain expression between flies from the African and European populations, with the inter-population divergence being highly correlated between males and females. The differentially expressed genes included those involved in stress response, olfaction, and detoxification. Expression differences were associated with transposable element insertions at two genes implicated in insecticide resistance (Cyp6g1 and CHKov1). Analysis of the brain transcriptome revealed many genes differing in expression between populations that were not detected in previous studies using whole flies. There was little evidence for sex-specific regulatory adaptation in the brain, as most expression differences between populations were observed in both males and females. The enrichment of genes with sexually dimorphic expression on the X chromosome is consistent with dosage compensation mechanisms affecting sex-biased expression in somatic tissues.
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DOI：
10.1006/bbrc.2001.5067
发表时间：
2001-06-22
影响因子：
3.1
作者：
Ekengren, S;Hultmark, D
通讯作者：
Hultmark, D
DOI：
10.1016/j.cub.2010.06.076
发表时间：
2010-08-24
期刊：
Current biology : CB
影响因子：
--
作者：
Bachtrog D;Toda NR;Lockton S
通讯作者：
Lockton S
影响因子：
64.8
作者：
Graveley BR;Brooks AN;Carlson JW;Duff MO;Landolin JM;Yang L;Artieri CG;van Baren MJ;Boley N;Booth BW;Brown JB;Cherbas L;Davis CA;Dobin A;Li R;Lin W;Malone JH;Mattiuzzo NR;Miller D;Sturgill D;Tuch BB;Zaleski C;Zhang D;Blanchette M;Dudoit S;Eads B;Green RE;Hammonds A;Jiang L;Kapranov P;Langton L;Perrimon N;Sandler JE;Wan KH;Willingham A;Zhang Y;Zou Y;Andrews J;Bickel PJ;Brenner SE;Brent MR;Cherbas P;Gingeras TR;Hoskins RA;Kaufman TC;Oliver B;Celniker SE
通讯作者：
Celniker SE
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
4.5
作者：
Albert FW;Somel M;Carneiro M;Aximu-Petri A;Halbwax M;Thalmann O;Blanco-Aguiar JA;Plyusnina IZ;Trut L;Villafuerte R;Ferrand N;Kaiser S;Jensen P;Pääbo S
通讯作者：
Pääbo S