Anti-inflammatory effects of shikonin in human periodontal ligament cells.
Anti-inflammatory effects of shikonin in human periodontal ligament cells.
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DOI：
10.1080/13880209.2018.1506482
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发表时间：
2018-12
影响因子：
3.8
通讯作者：
Upton Z
中科院分区：
文献类型：
--
作者：
Fan C;Zhang X;Upton Z
背景：紫草素是一种从紫草中提取的活性成分，已有报道在多种细胞中具有抗炎作用。然而，其对脂多糖(LPS)刺激的人牙周韧带细胞(HPDLCs)的影响尚不清楚。目的：探讨SHI对内毒素刺激的人肺泡巨噬细胞(HPDLCs)炎症相关细胞因子表达的影响。材料和方法：采用四甲基偶氮唑盐(3-(4，5-dimethylthiazol-2-yl)-2，5-diphenyltetrazolium)比色法检测SHI(0.125.0 5、0.2 5、0.5、1、2 μg/m L)作用1、3、7 后对人PDLCs增殖的影响。采用定量逆转录聚合酶链式反应(QRT-α)检测SHI(0.2 5和0.5 μg/m L)作用下人PDLCs中IL-1、IL-6、肿瘤坏死因子-2(TN-α)、基质金属蛋白酶-2(MMP2)、MMP9和COX-2的表达。Western blotting检测SHI在hPDLC中触发的信号转导通路。结果：SHI对人肺小管上皮细胞的LD_(50)为1.7 μg/m L(第1天)和1.1 μg/m L(第3天和第7天)。单纯脂多糖(1 μg/m L)或脂多糖加SHI(0.2 5和0.5 μg/m L)处理后，hPDLCs的形态无明显变化。定量逆转录聚合酶链式反应结果表明，SHI可抑制脂多糖诱导的人肺小管上皮细胞IL-1、IL-6、肿瘤坏死因子-α、基质金属蛋白酶-2、基质金属蛋白酶-9和环氧合酶-2的升高。HPDLCs经0.25或0.5κg/mLSHI处理后，磷酸化细胞外信号调节激酶和核因子-κB表达下调，I-κB表达上调。讨论与结论：SHI通过磷酸化ERK和NF-κB/I-κB信号通路对脂多糖刺激的牙周炎细胞具有抗炎作用，提示SHI可能是一种潜在的抗炎药物。
Context: Shikonin (SHI), an active component extracted from Radix Arnebiae, has been reported to possess anti-inflammatory properties in various cells. However, its effect on lipopolysaccharide (LPS)-stimulated human periodontal ligament cells (hPDLCs) is unknown. Objective: To investigate the effects of SHI on the expression of inflammatory related cytokines in LPS-stimulated hPDLCs. Materials and methods: The effects of SHI (0.125, 0.25, 0.5, 1, and 2 μg/mL) on hPDLCs proliferation for 1, 3 and 7 days were measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The expression of interleukin-1 (IL-1), IL-6, tumor necrosis factor-α (TNF-α), matrix metalloproteinase-2 (MMP-2), MMP-9 and cyclooxygenase-2 (COX-2) were detected in hPDLCs following SHI treatment (0.25 and 0.5 μg/mL) using Quantitative Reverse Transcriptase Polymerase Chain Reaction (qRT-PCR). The signaling pathways triggered by SHI in hPDLC were evaluated using western blotting. Results: LD50 of SHI is 1.7 μg/mL (day 1) and 1.1 μg/mL (day 3 and 7) in hPDLCs. No morphological changes were observed when hPDLCs were treated with LPS only (1 μg/mL) or LPS with SHI (0.25 and 0.5 μg/mL). Data from qRT-PCR suggests that SHI attenuates LPS-induced increases of IL-1, IL-6, TNF-α, MMP-2, MMP-9 and COX-2 in hPDLCs. Down-regulation of phosphorylated extracellular signal-regulated kinase (ERK) and nuclear factor-κB (NF-κB), and up-regulation of I-κB, were observed in LPS-stimulated hPDLCs after exposed to SHI at 0.25 or 0.5 μg/mL. Discussion and conclusions: SHI possesses anti-inflammatory effects in LPS-stimulated hPDLCs via phospho-ERK and NF-κB/I-κB signaling pathways; this suggests that SHI may hold potential as an anti-inflammatory agent against periodontitis.
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影响因子：
3.2
作者：
Goncalves, Davi C.;Evangelista, Rakel C.;Oria, Reinaldo B.
通讯作者：
Oria, Reinaldo B.
影响因子：
3.5
作者：
Mendes, R. T.;Sordi, R.;Fernandes, D.
通讯作者：
Fernandes, D.
影响因子：
2
作者：
Kim, Young Ock;Hong, Seung Jae;Yim, Sung-Vin
通讯作者：
Yim, Sung-Vin
影响因子：
7.3
作者：
Lee, Chen-Chen;Wang, Chien-Neng;Cheng, Yu-Wen
通讯作者：
Cheng, Yu-Wen
影响因子：
3.8
作者：
Min, R.;Zun, Z.;Chenping, Z.
通讯作者：
Chenping, Z.