A quiescent cell population replenishes mesenchymal stem cells to drive accelerated growth in mouse incisors.
A quiescent cell population replenishes mesenchymal stem cells to drive accelerated growth in mouse incisors.
复制标题
静止的细胞种群补充间充质干细胞以驱动小鼠切牙的加速生长。
DOI：
10.1038/s41467-017-02785-6
复制
发表时间：
2018-01-25
影响因子：
16.6
通讯作者：
Sharpe PT
中科院分区：
文献类型：
--
作者：
An Z;Sabalic M;Bloomquist RF;Fowler TE;Streelman T;Sharpe PT
间充质干细胞(MSC)群体的异质性在多大程度上与功能相关尚不清楚。利用MSC在体外的原型表面标记CD90/Thy1，我们发现在持续生长的小鼠中，30%的MSCs表达CD90/Thy1，这些细胞在出生后发育过程中产生了30%的分化细胞后代。在成年期，当生长速率动态平衡建立后，CD90/Thy1+MSCs数量急剧减少。当成人门牙被切断时，生长速度会增加，以迅速重建牙齿长度和动态平衡。这种加速的生长速度与CD90/Thy+MSCs的重新出现和它们对细胞分化的贡献的重新确立有关。一群Celsr1+的静止细胞在剪裁后变得有丝分裂，并补充CD90/Thy1群体。因此，在小鼠切牙中存在一个MSCs亚群，其区别在于CD90/Thy1的表达仅在生长速度加快的时期发挥特殊作用。小鼠的门牙不断地从缓慢循环的间充质干细胞群体中更新。在这里，作者表明，在切割成人门牙后，牙间充质干细胞的亚群重新激活，允许更快的生长速度和快速再生。
The extent to which heterogeneity within mesenchymal stem cell (MSC) populations is related to function is not understood. Using the archetypal MSC in vitro surface marker, CD90/Thy1, here we show that 30% of the MSCs in the continuously growing mouse incisor express CD90/Thy1 and these cells give rise to 30% of the differentiated cell progeny during postnatal development. In adulthood, when growth rate homeostasis is established, the CD90/Thy1+ MSCs decrease dramatically in number. When adult incisors are cut, the growth rate increases to rapidly re-establish tooth length and homeostasis. This accelerated growth rate correlates with the re-appearance of CD90/Thy+ MSCs and re-establishment of their contribution to cell differentiation. A population of Celsr1+ quiescent cells becomes mitotic following clipping and replenishes the CD90/Thy1 population. A sub-population of MSCs thus exists in the mouse incisor, distinguished by expression of CD90/Thy1 that plays a specific role only during periods of increased growth rate. Mouse incisors constantly renew from a slow cycling population of mesenchymal stem cells. Here, the authors show that upon cutting of adult incisors, a sub-population of dental mesenchymal stem cells reactivates, allowing an increased growth rate and rapid regeneration.
登录
查看更多内容
影响因子：
2.7
作者：
Fraser, Gareth J.;Bloomquist, Ryan F.;Streelman, J. Todd
通讯作者：
Streelman, J. Todd
影响因子：
64.5
作者：
Sugimura R;He XC;Venkatraman A;Arai F;Box A;Semerad C;Haug JS;Peng L;Zhong XB;Suda T;Li L
通讯作者：
Li L
影响因子：
23.9
作者：
Zhou, Bo O.;Yue, Rui;Murphy, Malea M.;Peyer, James G.;Morrison, Sean J.
通讯作者：
Morrison, Sean J.
影响因子：
23.9
作者：
Zhao, Hu;Feng, Jifan;Seidel, Kerstin;Shi, Songtao;Klein, Ophir;Sharpe, Paul;Chai, Yang
通讯作者：
Chai, Yang
影响因子：
64.5
作者：
Kretzschmar, Kai;Watt, Fiona M.
通讯作者：
Watt, Fiona M.