Intravital imaging-based analysis tools for vessel identification and assessment of concurrent dynamic vascular events.
Intravital imaging-based analysis tools for vessel identification and assessment of concurrent dynamic vascular events.
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DOI：
10.1038/s41467-018-04929-8
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发表时间：
2018-07-16
影响因子：
16.6
通讯作者：
Claesson-Welsh L
中科院分区：
文献类型：
--
作者：
Honkura N;Richards M;Laviña B;Sáinz-Jaspeado M;Betsholtz C;Claesson-Welsh L
在特定的刺激下，血管的直径、通透性和血流会发生变化。不同血管中这些反应的动力学和相互依赖性在很大程度上是未知的。在这里，我们报告了一种非侵入性技术，通过多光子显微镜和图像分析工具RVDM（相对速度，方向和形态）研究不同血管类别的动态事件，允许通过它们的红细胞（RBC）参数识别血管类别。此外，Claudin5启动子驱动的绿色荧光蛋白（GFP）表达用于区分毛细血管亚型。皮内注射血管内皮生长因子A （VEGFA）可诱导循环葡聚糖从缺乏GFP表达的毛细血管和小静脉渗漏，具有血管类型依赖的动力学。vegfa诱导的毛细血管渗漏与血管扩张和流速降低相吻合。因此，非侵入性刺激的活体成像与RVDM分析相结合，可以记录和量化脉管系统中非常快速的事件。不同的刺激可引起血流速度、血管直径和通透性的动态变化。在这里，作者开发了一种基于多光子显微镜的图像分析工具，可以识别血管并评估大血管网络的快速变化。
The vasculature undergoes changes in diameter, permeability and blood flow in response to specific stimuli. The dynamics and interdependence of these responses in different vessels are largely unknown. Here we report a non-invasive technique to study dynamic events in different vessel categories by multi-photon microscopy and an image analysis tool, RVDM (relative velocity, direction, and morphology) allowing the identification of vessel categories by their red blood cell (RBC) parameters. Moreover, Claudin5 promoter-driven green fluorescent protein (GFP) expression is used to distinguish capillary subtypes. Intradermal injection of vascular endothelial growth factor A (VEGFA) is shown to induce leakage of circulating dextran, with vessel-type-dependent kinetics, from capillaries and venules devoid of GFP expression. VEGFA-induced leakage in capillaries coincides with vessel dilation and reduced flow velocity. Thus, intravital imaging of non-invasive stimulation combined with RVDM analysis allows for recording and quantification of very rapid events in the vasculature. Different stimuli can induce dynamic changes in blood flow velocity, vessel diameter and permeability. Here the authors develop a multi-photon microscopy-based image analysis tool allowing the identification of vessels and the assessment of rapid changes in large vascular networks.
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影响因子：
4
作者：
Dejana, Elisabetta;Orsenigo, Fabrizio;Lampugnani, Maria Grazia
通讯作者：
Lampugnani, Maria Grazia
影响因子：
10.8
作者：
Bates DO
通讯作者：
Bates DO
影响因子：
4.6
作者：
Egawa, Gyohei;Nakamizo, Satoshi;Natsuaki, Yohei;Doi, Hiromi;Miyachi, Yoshiki;Kabashima, Kenji
通讯作者：
Kabashima, Kenji
影响因子：
9.8
作者：
Nagy, Janice A.;Benjamin, Laura;Zeng, Huiyan;Dvorak, Ann M.;Dvorak, Harold F.
通讯作者：
Dvorak, Harold F.
影响因子：
5.5
作者：
CURRY, FE;MASON, JC;MICHEL, CC
通讯作者：
MICHEL, CC