Effect of Gender to Fat Deposition in Yaks Based on Transcriptomic and Metabolomics Analysis.
Effect of Gender to Fat Deposition in Yaks Based on Transcriptomic and Metabolomics Analysis.
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DOI：
10.3389/fcell.2021.653188
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发表时间：
2021
影响因子：
5.5
通讯作者：
Guo X
中科院分区：
文献类型：
--
作者：
Xiong L;Pei J;Wu X;Kalwar Q;Yan P;Guo X
牦牛脂肪沉积对牦牛的生存、繁殖和肉质具有重要的影响。本研究采用mRNA-Seq和非靶向代谢组学方法，探讨了公牦牛和母牦牛脂肪沉积的特点以及性别对牦牛脂肪沉积的调控。FYs具有较高的内脏脂肪率、背最长肌和肝脏脂肪含量以及皮下脂肪厚度（p < 0.05）。肝脏脂肪和胆固醇合成增加，血液脂肪转运增加。牦牛的脂肪代谢是碳水化合物、脂肪酸和氨基酸代谢通过三羧酸（TCA）循环共同作用的结果，甘油三酯（TG）合成的增加伴随着类固醇合成的增加。高水平的肌醇和皮质醇（COR）（p < 0.01）激活了FY皮下脂肪中的钙信号，随后脂肪细胞分泌增加，并导致更多的瘦素（LEP）分泌（p < 0.01）。然后通过粘着斑和ECM-受体相互作用激活过氧化物酶体增殖物激活受体（PPAR）信号。最后，TG和类固醇合成通过ME 1、SCD、DIGVL 6、DGAT 2、DBI、LPL、CPT 1、PLIN 1、利帕、DHCR 24和SQLE基因的表达调控而增加。上述基因可作为今后牦牛高脂性状分子育种的候选基因。本研究可为牦牛肉质改良及育种提供理论依据。
Fat deposition in yaks plays an important part in survival, multiplication, and meat quality. In this work, the characteristic of fat deposition in male yaks (MYs) and female yaks (FYs) and the regulations of gender to yak fat deposition were explored by mRNA-Seq and non-targeted metabolomics analyses. FYs possessed a higher body fat rate (BFR) of visceral fat, fat content in longissimus dorsi (LD) and liver, and subcutaneous fat thickness (p < 0.05). The fat and cholesterol synthesis in liver and the fat transport in FY blood increased. The fat metabolism in yaks is the combined effect of carbohydrate, fatty acid, and amino acid metabolism by tricarboxylic acid (TCA) cycle, and an increase of triglyceride (TG) synthesis was accompanied by an increase of steroid synthesis. The high levels of myo-inositol and cortisol (COR) (p < 0.01) activated the calcium signaling in FY subcutaneous fat, followed by the increase of adipocyte secretion, and resulted in more leptin (LEP) secretion (p < 0.01). Then peroxisome proliferator-activated receptor (PPAR) signaling was activated by the focal adhesions and ECM–receptor interaction. Finally, the TG and steroid synthesis increased by the expression regulation of ME1, SCD, ELOVL6, DGAT2, DBI, LPL, CPT1, PLIN1, LIPA, DHCR24, and SQLE gene. The above genes can be considered as the candidate genes for yak with higher fat amount in molecular breeding in the future. This study can provide a theoretical basis for improving the meat quality and breeding of yaks.
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影响因子：
3.3
作者：
Jeong, J.;Bong, J.;Baik, M.
通讯作者：
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影响因子：
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Portillo MP
影响因子：
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作者：
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通讯作者：
Beriain, Maria Jose