Elevated humoral immune response to heat shock protein 60 (hsp60) family in periodontitis patients
Elevated humoral immune response to heat shock protein 60 (hsp60) family in periodontitis patients
复制标题
牙周炎患者对热休克蛋白 60 (hsp60) 家族的体液免疫反应升高
DOI：
--
复制
发表时间：
2000
影响因子：
4.6
通讯作者：
Kenshiro Hara
中科院分区：
文献类型：
--
作者：
K. Tabeta;K. Yamazaki;H. Hotokezaka;H. Yoshie;Kenshiro Hara
检查了牙周炎患者血清和发炎牙龈组织中60 kD人和牙龈卟啉单胞菌GroEL hsp60抗体的存在。为了获得抗原，构建了携带人hsp60和牙龈卟啉单胞菌GroEL基因的重组质粒，并表达为组氨酸标记的重组蛋白。 这些蛋白质的免疫反应性通过对哺乳动物hsp60具有特异性并与哺乳动物和细菌hsp60交叉反应的MoAb证实。Western印迹分析清楚地表明，牙周炎患者对牙龈卟啉单胞菌GroEL显示阳性反应的数量高于牙周健康受试者的数量。 此外，在所有牙龈组织提取物样本中均检测到抗牙龈卟啉单胞菌GroEL抗体。 对于人类热休克蛋白60，发现在牙周炎患者血清阳性的频率比在健康受试者。此外，牙周炎患者表现出更强的反应性相比，健康受试者。血清抗体的ELISA定量分析也表明，患者血清中的抗体水平显着高于对照组。在牙龈组织提取物中，10名患者中有7名表现出对人热休克蛋白60的阳性反应，其中10名表现出强阳性。来自选定患者的针对人hsp60和牙龈卟啉单胞菌GroEL的亲和纯化血清抗体分别与牙龈卟啉单胞菌GroEL和人hsp60反应，表明抗体的交叉反应性。 这些结果表明，牙周致病菌牙龈卟啉单胞菌的GroEL和自体人热休克蛋白60之间的分子模拟可能在牙周炎的免疫机制中发挥一定作用。
The presence of antibodies to the 60‐kD human and Porphyromonas gingivalis GroEL hsp60 in the sera and inflamed gingival tissues of periodontitis patients was examined. In order to obtain the antigens, recombinant plasmids carrying human hsp60 and P. gingivalis GroEL genes were constructed and expressed as histidine‐tagged recombinant proteins. Immunoreactivities of these proteins were confirmed by MoAbs specific to mammalian hsp60 and cross‐reactive with both mammalian and bacterial hsp60. Western blot analysis clearly demonstrated that the number of periodontitis patients showing a positive response to P. gingivalis GroEL was higher than the number of periodontally healthy subjects. Furthermore, anti‐P. gingivalis GroEL antibody was detected in all samples of gingival tissue extracts. For human hsp60, a higher frequency of seropositivity was found in the periodontitis patients than in the healthy subjects. In addition, the periodontitis patients demonstrated stronger reactivity compared with the healthy subjects. Quantitative analysis of serum antibodies by ELISA also demonstrated that the levels of antibodies in the sera of patients were significantly higher than those of control subjects. In the gingival tissue extracts, seven out of 10 patients demonstrated a positive response to human hsp60 and tso of these demonstrated strong positivity. Affinity‐purified serum antibodies to human hsp60 and P. gingivalis GroEL from selected patients reacted with P. gingivalis GroEL and human hsp60, respectively, suggesting cross‐reactivity of antibodies. These results suggest that molecular mimicry between GroEL of the periodontopathic bacterium P. gingivalis and autologous human hsp60 may play some role in immune mechanisms in periodontitis.
成人牙周病中胶原蛋白的自身免疫。
DOI：
10.1111/j.1600-0714.1988.tb01315.x
发表时间：
1988
期刊：
Journal of oral pathology
影响因子：
--
作者：
Hirsch,HZ;Tarkowski,A;Miller,EJ;Gay,S;Koopman,WJ;Mestecky,J
通讯作者：
Mestecky,J
多克隆 B 细胞激活剂以及体外诱导与胶原蛋白发生反应的自身抗体。
影响因子：
3.5
作者：
Hahn,CL;Schenkein,HA;Tew,JG
通讯作者：
Tew,JG
与牙周病相关的 5 种细菌的自然分布。
影响因子：
6.7
作者：
Wolff,LF;Aeppli,DM;Pihlstrom,B;Anderson,L;Stoltenberg,J;Osborn,J;Hardie,N;Shelburne,C;Fischer,G
通讯作者：
Fischer,G