The use of micropatterning to control smooth muscle myosin heavy chain expression and limit the response to transforming growth factor β1 in vascular smooth muscle cells.
The use of micropatterning to control smooth muscle myosin heavy chain expression and limit the response to transforming growth factor β1 in vascular smooth muscle cells.
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DOI：
10.1016/j.biomaterials.2010.08.105
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发表时间：
2011-01
期刊：
影响因子：
14
通讯作者：
Wong, Joyce Y.
中科院分区：
文献类型：
--
作者：
Williams, Corin;Brown, Xin Q.;Bartolak-Suki, Erzsebet;Ma, Hongwei;Chilkoti, Ashutosh;Wong, Joyce Y.
在健康动脉中，收缩性血管平滑肌细胞 (VSMC) 具有细长的形状，高度排列，但在培养物中转变为合成表型，同时变得分布良好且随机组织。因此，控制 VSMC 表型是组织工程中的一个挑战。在这项研究中，我们研究了微图案化对低传代和高传代以及存在转化生长因子β1 (TGFβ1) 的VSMC 中收缩蛋白表达的影响。与无图案的 VSMC 相比，微图案化导致细胞面积显着减小，伸长率增加，排列增加，与传代次数无关。在血清存在的情况下，微图案化导致低传代VSMC中平滑肌肌球蛋白重链（SM-MHC）和α-肌动蛋白表达增加，但对高传代VSMC没有影响。微图案在低传代时上调 SM-MHC 与 TGFβ1 一样有效；然而，微图案化限制了低传代和高传代时 VSMC 对 TGFβ1 的反应。对 TGFβ 受体 1 的研究表明，与高传代时有图案的 VSMC 相比，无图案 VSMC 中的表达量更高。我们的研究表明，微图案化是收缩性 VSMC 中 SM-MHC 表达的重要调节因子，并且它可能提供生长因子存在下表型稳定的机制。
In the healthy artery, contractile vascular smooth muscle cells (VSMCs) have an elongated shape and are highly aligned but transition to a synthetic phenotype in culture, while additionally becoming well spread and randomly organized. Thus, controlling VSMC phenotype is a challenge in tissue engineering. In this study, we investigated the effects of micropatterning on contractile protein expression in VSMCs at low and high passage and in the presence of transforming growth factor beta 1 (TGFβ1). Micropatterning led to significantly decreased cell area, increased elongation, and increased alignment compared to non-patterned VSMCs independent of passage number. In the presence of serum, micropatterning led to increased smooth muscle myosin heavy chain (SM-MHC) and α-actin expression in low passage VSMCs, but had no effect on high passage VSMCs. Micropatterning was as effective as TGFβ1 in up-regulating SM-MHC at low passage; however, micropatterning limited VSMC response to TGFβ1 at both low and high passage. Investigation of TGFβ receptor 1 revealed higher expression in non-patterned VSMCs compared to patterned at high passage. Our studies demonstrate that micropatterning is an important regulator of SM-MHC expression in contractile VSMCs and that it may provide a mechanism for phenotype stabilization in the presence of growth factors.
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影响因子：
20.1
作者：
Li, SH;Sims, S;Pickering, JG
通讯作者：
Pickering, JG
DOI：
10.1083/jcb.89.2.379
发表时间：
1981-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Chamley-Campbell JH;Campbell GR;Ross R
通讯作者：
Ross R
DOI：
10.1161/01.atv.11.3.681
发表时间：
1991-05-01
期刊：
ARTERIOSCLEROSIS AND THROMBOSIS
影响因子：
--
作者：
FINLAY, HM;DIXON, JG;CANHAM, PB
通讯作者：
CANHAM, PB
影响因子：
14
作者：
Cao, Ye;Poon, Yin Fun;Chan-Park, Mary B.
通讯作者：
Chan-Park, Mary B.
影响因子：
6.6
作者：
Rama, Aisha;Matsushita, Tsutomu;Severs, Nicholas J.
通讯作者：
Severs, Nicholas J.