Genetic structure of Plasmodium vivax and Plasmodium falciparum in the Bannu district of Pakistan.
Genetic structure of Plasmodium vivax and Plasmodium falciparum in the Bannu district of Pakistan.
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DOI：
10.1186/1475-2875-9-112
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发表时间：
2010-04-23
期刊：
影响因子：
3
通讯作者：
Yan G
中科院分区：
文献类型：
--
作者：
Khatoon L;Baliraine FN;Bonizzoni M;Malik SA;Yan G
间日疟原虫和恶性疟原虫是疟疾的主要病原体。虽然了解疟疾寄生虫的遗传结构有助于了解寄生虫毒力的演变、设计抗疟疾疫苗和评估疟疾控制措施的影响，但巴基斯坦缺乏关于这两种疟疾寄生虫遗传多样性的信息。本研究旨在阐明间日疟原虫和恶性疟原虫在这一研究不足地区的遗传结构。采用聚合酶链反应- rflp技术对巴基斯坦人口稠密的疟疾流行地区班努地区的间日疟原虫和恶性疟原虫进行遗传多样性分析。其中，对间日疟原虫的Pvmsp-3α和Pvmsp-3β基因以及恶性疟原虫的Pfmsp-1和Pfmsp-2基因进行分析。在间日疟原虫中，Pvmsp-3α和Pvmsp-3β基因分型在这些位点显示出高度的多样性。Pvmsp-3α检测到A （1.9 kb）、B （1.5 kb）、C （1.2 kb）和D (0.3 kb) 4个不同的等位基因群，其中A型最多（82%）。相反，间日疟原虫msp-3β基因座扩增产生两个等位基因群：A （1.7 ~ 2.2 kb, 62%）和B (1.4 ~ 1.5 kb, 33%)，混合菌株感染率为5%。对Pvmsp-3α和Pvmsp-3β进行限制性内切分析，分别得到12个和8个不同的等位基因，混合基因型组合患病率为20%。在恶性疟原虫中，所有已知的Pfmsp-1基因型和Pfmsp-2基因型均被检测到，MAD20在67%的分离株中出现，3D7/IC在65%的分离株中出现。总体而言，24%的恶性疟原虫样本出现混合菌株感染。这些结果表明，巴基斯坦的间日疟原虫和恶性疟原虫种群高度多样化。
Plasmodium vivax and Plasmodium falciparum are the major causative agents of malaria. While knowledge of the genetic structure of malaria parasites is useful for understanding the evolution of parasite virulence, designing anti-malarial vaccines and assessing the impact of malaria control measures, there is a paucity of information on genetic diversity of these two malaria parasites in Pakistan. This study sought to shed some light on the genetic structure of P. vivax and P. falciparum in this understudied region. The genetic diversities of P. vivax and P. falciparum populations from the densely populated, malaria-endemic Bannu district of Pakistan were evaluated by analysis of their merozoite surface protein (msp) genes by PCR-RFLP. Specifically, the Pvmsp-3α and Pvmsp-3β genes of P. vivax and the Pfmsp-1 and Pfmsp-2 genes of P. falciparum were analysed. In P. vivax, genotyping of Pvmsp-3α and Pvmsp-3β genes showed a high level of diversity at these loci. Four distinct allele groups: A (1.9 kb), B (1.5 kb), C (1.2 kb), and D (0.3 kb) were detected for Pvmsp-3α, type A being the most prevalent (82%). Conversely, amplification of the P. vivax msp-3β locus produced two allele groups: A (1.7-2.2 kb, 62%) and B (1.4-1.5 kb, 33%), with 5% mixed-strain infections. Restriction analysis of Pvmsp-3α and Pvmsp-3β yielded 12 and 8 distinct alleles, respectively, with a combined mixed genotype prevalence of 20%. In P. falciparum, all three known genotypes of Pfmsp-1 and two of Pfmsp-2 were observed, with MAD20 occurring in 67% and 3D7/IC in 65% of the isolates, respectively. Overall, 24% P. falciparum samples exhibited mixed-strain infections. These results indicate that both P. vivax and P. falciparum populations in Pakistan are highly diverse.
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影响因子：
64.8
作者：
Carlton, Jane M.;Adams, John H.;Silva, Joana C.;Bidwell, Shelby L.;Lorenzi, Hernan;Caler, Elisabet;Crabtree, Jonathan;Angiuoli, Samuel V.;Merino, Emilio F.;Amedeo, Paolo;Cheng, Qin;Coulson, Richard M. R.;Crabb, Brendan S.;del Portillo, Hernando A.;Essien, Kobby;Feldblyum, Tamara V.;Fernandez-Becerra, Carmen;Gilson, Paul R.;Gueye, Amy H.;Guo, Xiang;Kang'a, Simon;Kooij, Taco W. A.;Korsinczky, Michael;Meyer, Esmeralda V. -S.;Nene, Vish;Paulsen, Ian;White, Owen;Ralph, Stuart A.;Ren, Qinghu;Sargeant, Tobias J.;Salzberg, Steven L.;Stoeckert, Christian J.;Sullivan, Steven A.;Yamamoto, Marcio M.;Hoffman, Stephen L.;Wortman, Jennifer R.;Gardner, Malcolm J.;Galinski, Mary R.;Barnwell, John W.;Fraser-Liggett, Claire M.
通讯作者：
Fraser-Liggett, Claire M.
DOI：
10.4269/ajtmh.2003.68.613
发表时间：
2003-05-01
影响因子：
3.3
作者：
Cui, LW;Mascorro, CN;Sattabongkot, J
通讯作者：
Sattabongkot, J
影响因子：
1.5
作者：
Galinski, MR;Ingravallo, P;Barnwell, JW
通讯作者：
Barnwell, JW
影响因子：
3
作者：
Joshi, Hema;Valecha, Neena;Dash, Aditya P.
通讯作者：
Dash, Aditya P.
影响因子：
2.1
作者：
Kumar, SV;Ranjan, S;Das, A
通讯作者：
Das, A