Collection of SARS-CoV-2 Virus from the Air of a Clinic Within a University Student Health Care Center and Analyses of the Viral Genomic Sequence.
Collection of SARS-CoV-2 Virus from the Air of a Clinic Within a University Student Health Care Center and Analyses of the Viral Genomic Sequence.
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DOI：
10.4209/aaqr.2020.02.0202
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发表时间：
2020-06
影响因子：
4
通讯作者：
Wu CY
中科院分区：
文献类型：
--
作者：
Lednicky JA;Shankar SN;Elbadry MA;Gibson JC;Alam MM;Stephenson CJ;Eiguren-Fernandez A;Morris JG;Mavian CN;Salemi M;Clugston JR;Wu CY
通过识别SARS-CoV-2基因组序列中随时间发生的突变，可以跟踪全球COVID-19的进展。这些努力目前依赖于对患者标本中的SARS-CoV-2基因组进行测序（直接测序）或对从细胞培养物中的患者标本中分离的病毒进行测序。在一所大学学生保健中心的诊所进行的SARS-CoV-2空气采样试验研究检测到病毒vRNA，估计浓度为0.87病毒基因组L−1空气。为了确定检测到的病毒是否是活的（“活的”），尝试在细胞培养物中分离病毒。用来自空气样品的收集培养基接种Vero E6细胞后两天内观察到病毒诱导的细胞病变效应（CPE）;然而，来自细胞培养物的SARS-CoV-2 vRNA的rtRT-PCR试验为阴性。相反，其他三种快速生长的人类呼吸道病毒被分离出来，随后被鉴定出来，这说明了当测试样本中存在多种病毒时，分离SARS-CoV-2的挑战。然而，通过桑格测序确定了完整的SAR-CoV-2基因组序列，并且与先前在美国格鲁吉亚描述的SAR-CoV-2基因组最相似。这项研究的结果说明了使用环境气溶胶样本而不是人体样本跟踪COVID-19大流行的可行性。从距离最近的患者交通超过2米的距离收集阳性样本意味着病毒存在于气溶胶中。
The progression of COVID-19 worldwide can be tracked by identifying mutations within the genomic sequence of SARS-CoV-2 that occur as a function of time. Such efforts currently rely on sequencing the genome of SARS-CoV-2 in patient specimens (direct sequencing) or of virus isolated from patient specimens in cell cultures. A pilot SARS-CoV-2 air sampling study conducted at a clinic within a university student health care center detected the virus vRNA, with an estimated concentration of 0.87 virus genomes L−1 air. To determine whether the virus detected was viable (‘live’), attempts were made to isolate the virus in cell cultures. Virus-induced cytopathic effects (CPE) were observed within two days post-inoculation of Vero E6 cells with collection media from air samples; however, rtRT-PCR tests for SARS-CoV-2 vRNA from cell culture were negative. Instead, three other fast-growing human respiratory viruses were isolated and subsequently identified, illustrating the challenge in isolating SARS-CoV-2 when multiple viruses are present in a test sample. The complete SAR-CoV-2 genomic sequence was nevertheless determined by Sanger sequencing and most closely resembles SARS-CoV-2 genomes previously described in Georgia, USA. Results of this study illustrate the feasibility of tracking progression of the COVID-19 pandemic using environmental aerosol samples instead of human specimens. Collection of a positive sample from a distance more than 2 m away from the nearest patient traffic implies the virus was in an aerosol.
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影响因子：
4
作者：
Hsiao, Ta-Chih;Chuang, Hsiao-Chi;Young, Li-Hao
通讯作者：
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影响因子：
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作者：
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影响因子：
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作者：
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DOI：
10.1073/pnas.2004999117
发表时间：
2020-04-28
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
4
作者：
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通讯作者：
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