Drosophila polo kinase is required for cytokinesis.
Drosophila polo kinase is required for cytokinesis.
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DOI：
10.1083/jcb.143.3.659
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发表时间：
1998-11-02
影响因子：
7.8
通讯作者：
Glover, DM
中科院分区：
文献类型：
--
作者：
Carmena, M;Riparbelli, MG;Minestrini, G;Tavares, AM;Adams, R;Callaini, G;Glover, DM
许多证据表明，果蝇 polo 基因的亚等位基因在精子发生过程中胞质分裂缺陷占主导地位。在减数分裂前的有丝分裂中，胞质分裂缺陷导致初级精母细胞囊肿，其中可含有多个中心体的细胞数量减少。它们通过相应减少数量的环管连接，环管是通过稳定卵裂沟形成的结构。减数分裂期间最早的缺陷是未能在末期形成正确的中区和中体结构。这伴随着未能正确定位在胞质分裂中发挥作用的帕瓦罗蒂驱动蛋白样蛋白，以及未能正确定位并入收缩环的隔膜花生和肌动蛋白。尽管存在这些缺陷，细胞周期蛋白 B 仍会被降解，细胞会退出 M 期。产生的精子细胞通常是双核或四核的，在这种情况下，它们分别发育出两个或四个轴丝。很大一部分胞质分裂失败的精子细胞也可能表现出先前归因于 polo1 突变体的分离缺陷。我们讨论了有关 Polo 样激酶在调节 M 期进展（包括胞质分裂的最早事件）中的保守功能的这些发现。
A number of lines of evidence point to a predominance of cytokinesis defects in spermatogenesis in hypomorphic alleles of the Drosophila polo gene. In the pre-meiotic mitoses, cytokinesis defects result in cysts of primary spermatocytes with reduced numbers of cells that can contain multiple centrosomes. These are connected by a correspondingly reduced number of ring canals, structures formed by the stabilization of the cleavage furrow. The earliest defects during the meiotic divisions are a failure to form the correct mid-zone and mid-body structures at telophase. This is accompanied by a failure to correctly localize the Pavarotti kinesin- like protein that functions in cytokinesis, and of the septin Peanut and of actin to be incorporated into a contractile ring. In spite of these defects, cyclin B is degraded and the cells exit M phase. The resulting spermatids are frequently binuclear or tetranuclear, in which case they develop either two or four axonemes, respectively. A significant proportion of spermatids in which cytokinesis has failed may also show the segregation defects previously ascribed to polo1 mutants. We discuss these findings in respect to conserved functions for the Polo-like kinases in regulating progression through M phase, including the earliest events of cytokinesis.
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影响因子：
9.2
作者：
Charles, JF;Jespersen, SL;Morgan, DO
通讯作者：
Morgan, DO
影响因子：
7.8
作者：
PESACRETA, TC;BYERS, TJ;BRANTON, D
通讯作者：
BRANTON, D
影响因子：
64.5
作者：
NEUFELD, TP;RUBIN, GM
通讯作者：
RUBIN, GM
影响因子：
7.8
作者：
Lane, HA;Nigg, EA
通讯作者：
Nigg, EA
DOI：
10.1016/s0022-5320(71)90020-7
发表时间：
1971-01-01
期刊：
JOURNAL OF ULTRASTRUCTURE RESEARCH
影响因子：
--
作者：
KALT, MR;TANDLER, B
通讯作者：
TANDLER, B