Mesothelial cell differentiation into osteoblast- and adipocyte-like cells.
Mesothelial cell differentiation into osteoblast- and adipocyte-like cells.
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间皮细胞分化为成骨细胞和脂肪细胞样细胞。
DOI：
10.1111/j.1582-4934.2010.01212.x
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发表时间：
2011-10
影响因子：
5.3
通讯作者：
Mutsaers SE
中科院分区：
文献类型：
--
作者：
Lansley SM;Searles RG;Hoi A;Thomas C;Moneta H;Herrick SE;Thompson PJ;Newman M;Sterrett GF;Prêle CM;Mutsaers SE
包括恶性间皮瘤（MM）在内的浆液性病变可显示骨和/或软骨分化的特征，尽管其形成机制尚不清楚。间皮细胞具有分化为具有肌成纤维细胞、平滑肌和内皮细胞特征的细胞的能力。它们是否能分化成其他类型的细胞尚不清楚。本研究验证了间皮细胞可以分化为胚胎中胚层的细胞谱系，包括成骨细胞和脂肪细胞的假设。为了检验这一点，在体外用原代大鼠和人间皮细胞进行骨形成的功能测定和脂肪形成测定，所述原代大鼠和人间皮细胞在成骨或脂肪形成培养基（AM）中维持0-26天。间皮细胞表达碱性磷酸酶水平增加，成骨细胞表型的早期标志物，并形成矿化骨样结节。当在AM中培养时，间皮细胞也积累指示成熟脂肪细胞表型的脂质。所有细胞均表达几种关键的成骨细胞和脂肪细胞标志物，包括成骨细胞特异性runt相关转录因子2，并表现出与上皮-间充质转化一致的mRNA表达变化。总之，这些研究证实，间皮细胞有能力分化成成骨细胞和脂肪细胞样细胞，提供了明确的证据，其多潜能的性质。这些数据强烈支持间皮细胞分化作为MM肿瘤和其他血清病理学中不同组织类型的潜在来源，并为间皮细胞在再生疗法中的使用提供支持。
Serosal pathologies including malignant mesothelioma (MM) can show features of osseous and/or cartilaginous differentiation although the mechanism for its formation is unknown. Mesothelial cells have the capacity to differentiate into cells with myofibroblast, smooth muscle and endothelial cell characteristics. Whether they can differentiate into other cell types is unclear. This study tests the hypothesis that mesothelial cells can differentiate into cell lineages of the embryonic mesoderm including osteoblasts and adipocytes. To examine this, a functional assay of bone formation and an adipogenic assay were performed in vitro with primary rat and human mesothelial cells maintained in osteogenic or adipogenic medium (AM) for 0–26 days. Mesothelial cells expressed increasing levels of alkaline phosphatase, an early marker of the osteoblast phenotype, and formed mineralized bone-like nodules. Mesothelial cells also accumulated lipid indicative of a mature adipocyte phenotype when cultured in AM. All cells expressed several key osteoblast and adipocyte markers, including osteoblast-specific runt-related transcription factor 2, and demonstrated changes in mRNA expression consistent with epithelial-to-mesenchymal transition. In conclusion, these studies confirm that mesothelial cells have the capacity to differentiate into osteoblast- and adipocyte-like cells, providing definitive evidence of their multipotential nature. These data strongly support mesothelial cell differentiation as the potential source of different tissue types in MM tumours and other serosal pathologies, and add support for the use of mesothelial cells in regenerative therapies.
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影响因子：
4.4
作者：
Decologne, Nathalie;Kolb, Martin;Bonniaud, Philippe
通讯作者：
Bonniaud, Philippe
影响因子：
2.9
作者：
Muñoz-Chápuli, R;Pérez-Pomares, JM;González, M
通讯作者：
González, M
Osf2/Cbfa1：成骨细胞分化的转录激活因子
影响因子：
64.5
作者：
Ducy, P;Zhang, R;Karsenty, G
通讯作者：
Karsenty, G
影响因子：
2.8
作者：
HANSEN, O;SIM, F;GRUNER, OPN
通讯作者：
GRUNER, OPN
影响因子：
4.2
作者：
BELLOWS, CG;AUBIN, JE;ANTOSZ, ME
通讯作者：
ANTOSZ, ME