Sulfated vizantin inhibits biofilm maturation by Streptococcus mutans
Sulfated vizantin inhibits biofilm maturation by Streptococcus mutans
复制标题
硫酸维赞汀抑制变形链球菌生物膜成熟
DOI：
10.1111/1348-0421.12797
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发表时间：
2020
影响因子：
2.6
通讯作者：
Terao Yutaka
中科院分区：
文献类型：
--
作者：
Oda Masataka;Kurosawa Mie;Yamamoto Hirofumi;Domon Hisanori;Takenaka Shoji;Ohsumi Tatsuya;Maekawa Tomoki;Yamasaki Naoto;Furue Yui;Terao Yutaka
变形链球菌是龋齿的主要病原体，通过细菌葡萄糖基转移酶产生的可溶性和不可溶性葡聚糖附着在牙齿表面。因此,酒店。突变糖基转移酶是这种致龋细菌的重要毒力因子。硫酸维赞汀有效抑制ys形成生物膜。在不影响其生长的情况下进行变形。在这项研究中，更少。涂有硫酸维辛丁的羟基磷灰石盘比未涂有硫酸维辛丁的羟基磷灰石盘更容易形成变形生物膜。硫酸酸化维赞汀对人牙龈细胞系Ca9‐22无细胞毒性。硫酸维赞汀剂量依赖性地抑制细胞外游离葡萄糖基转移酶的释放。与未经处理的细菌相比，Mutansand增强了细胞相关葡萄糖基转移酶的积累。硫酸酸化的维赞汀破坏了细胞相关葡萄糖基转移酶和细胞游离葡萄糖基转移酶之间的定位平衡，导致生物膜成熟受到抑制。这些结果表明，磺化维zantin有可能成为一种预防龋病的新型药物。
Streptococcus mutansis the main pathogen of dental caries and adheres to the tooth surface via soluble and insoluble glucans produced by the bacterial glucosyltransferase enzyme. Thus, theS. mutansglucosyltransferase is an important virulence factor for this cariogenic bacterium. Sulfated vizantin effectively inhibits biofilm formation byS. mutanswithout affecting its growth. In this study, lessS. mutansbiofilm formation occurred on hydroxyapatite discs coated with sulfated vizantin than on noncoated discs. Sulfated vizantin showed no cytotoxicity against the human gingival cell line Ca9‐22. Sulfated vizantin dose‐dependently inhibited the extracellular release of cell‐free glucosyltransferase fromS. mutansand enhanced the accumulation of cell‐associated glucosyltransferase, compared with that observed with untreated bacteria. Sulfated vizantin disrupted the localization balance between cell‐associated glucosyltransferase and cell‐free glucosyltransferase, resulting in inhibited biofilm maturation. These results indicate that sulfated vizantin can potentially serve as a novel agent for preventing dental caries.
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影响因子：
9.4
作者：
HAMADA, S;MASUDA, N;KOTANI, S
通讯作者：
KOTANI, S
影响因子：
4.4
作者：
A. Yoshida;T. Ansai;T. Takehara;H. Kuramitsu
通讯作者：
A. Yoshida;T. Ansai;T. Takehara;H. Kuramitsu
影响因子：
4.3
作者：
Kopec, LK;VaccaSmith, AM;Bowen, WH
通讯作者：
Bowen, WH
选定的表面活性剂对来自变形链球菌的纯化葡萄糖基转移酶和细胞粘附到光滑表面的影响。
影响因子：
3
作者：
Shigetada Kawabata;M. Torii;T. Minami;T. Fujiwara;Shigeyuki Hamada
通讯作者：
Shigeyuki Hamada
影响因子：
7.6
作者：
HAMADA, S;KOGA, T;OOSHIMA, T
通讯作者：
OOSHIMA, T