DAXX co-folds with H3.3/H4 using high local stability conferred by the H3.3 variant recognition residues.
DAXX co-folds with H3.3/H4 using high local stability conferred by the H3.3 variant recognition residues.
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DAXX与H3.3/H4共同使用H3.3变体识别残基赋予的高局部稳定性。
DOI：
10.1093/nar/gku090
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发表时间：
2014-04
影响因子：
14.9
通讯作者：
Black BE
中科院分区：
文献类型：
--
作者：
DeNizio JE;Elsässer SJ;Black BE
Histone chaperones are a diverse class of proteins that facilitate chromatin assembly. Their ability to stabilize highly abundant histone proteins in the cellular environment prevents non-specific interactions and promotes nucleosome formation, but the various mechanisms for doing so are not well understood. We now focus on the dynamic features of the DAXX histone chaperone that have been elusive from previous structural studies. Using hydrogen/deuterium exchange coupled to mass spectrometry (H/DX-MS), we elucidate the concerted binding-folding of DAXX with histone variants H3.3/H4 and H3.2/H4 and find that high local stability at the variant-specific recognition residues rationalizes its known selectivity for H3.3. We show that the DAXX histone binding domain is largely disordered in solution and that formation of the H3.3/H4/DAXX complex induces folding and dramatic global stabilization of both histone and chaperone. Thus, DAXX uses a novel strategy as a molecular chaperone that paradoxically couples its own folding to substrate recognition and binding. Further, we propose a model for the chromatin assembly reaction it mediates, including a stepwise folding pathway that helps explain the fidelity of DAXX in associating with the H3.3 variant, despite an extensive and nearly identical binding surface on its counterparts, H3.1 and H3.2.
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影响因子：
10.5
作者：
Drane, Pascal;Ouararhni, Khalid;Hamiche, Ali
通讯作者：
Hamiche, Ali
使用脉冲EPR光谱与位置定向的自旋标记探测（H3-H4）2组蛋白四聚体结构。
影响因子：
14.9
作者：
Bowman A;Ward R;El-Mkami H;Owen-Hughes T;Norman DG
通讯作者：
Norman DG
影响因子：
64.5
作者：
Goldberg AD;Banaszynski LA;Noh KM;Lewis PW;Elsaesser SJ;Stadler S;Dewell S;Law M;Guo X;Li X;Wen D;Chapgier A;DeKelver RC;Miller JC;Lee YL;Boydston EA;Holmes MC;Gregory PD;Greally JM;Rafii S;Yang C;Scambler PJ;Garrick D;Gibbons RJ;Higgs DR;Cristea IM;Urnov FD;Zheng D;Allis CD
通讯作者：
Allis CD
DOI：
10.1016/j.jasms.2006.06.006
发表时间：
2006-11-01
影响因子：
3.2
作者：
Englander, S. Walter
通讯作者：
Englander, S. Walter
影响因子：
16
作者：
Luk, Ed;Vu, Ngoc-Diep;Wu, Carl
通讯作者：
Wu, Carl