Prevalence of Campylobacter spp. in Raccoon Dogs and Badgers in Miyazaki Prefecture, Japan
Prevalence of Campylobacter spp. in Raccoon Dogs and Badgers in Miyazaki Prefecture, Japan
复制标题
弯曲杆菌属的流行率。
DOI：
10.1007/s10393-021-01527-x
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发表时间：
2021
期刊：
影响因子：
2.5
通讯作者：
N.
中科院分区：
文献类型：
--
作者：
Taniguchi;T.;Tarigan;E.;Sato;H.;Kaneko;C.;Misawa;N.
对日本宫崎县的28只浣熊犬和27只獾的55份肠内容物样品进行了弯曲杆菌的检测。jejuniandC.从浣熊犬中分离到的Uppsaliens分别为3.6%（n= 1）和75%（n= 21）。与此相反，没有弯曲杆菌属（Campylobacterspp.）是从獾中分离出来的C.对Uppsaliensisolates进行针对8种抗微生物剂的抗微生物敏感性测试。这表明，大多数从浣熊犬分离的菌株对所检测的抗菌药物敏感，而从宫崎县健康犬分离的菌株显示出较高的耐药性。结果表明，该菌存在毒力基因flaA、cadF、ciaB、cdtA、cdtB和cdtC。从一只浣熊狗身上分离出的空肠，除了从拉布拉多身上分离出的空肠。相比之下，所有这些毒力基因的检查都存在于所有C。分离自浣熊犬和犬的upsaliensisstrains。根据7个管家基因（adk、aspA、atpA、glnA、glyA、pgi、tkt）的核苷酸序列，对这些菌株的遗传多样性与C.从犬中分离的菌株和从人和犬中随机选择的菌株保存在弯曲杆菌MLST数据库中。系统发育树分析将浣熊犬与人、犬分离。这些结果表明，浣熊狗是C. upsaliens和分离株可能代表一个群体，从人类和狗不同。据我们所知，这是第一个研究表明，高患病率的C。在浣熊狗身上很敏感。
A total of 55 samples of intestinal contents from 28 raccoon dogs (Nyctereutes procyonoides) and 27 badgers (Males anakuma) in Miyazaki prefecture, Japan, were examined for the presence ofCampylobacterspecies.C. jejuniandC. upsaliensiswere isolated from 3.6% (n= 1) and 75% (n= 21) of raccoon dogs, respectively. In contrast, noCampylobacterspp. was isolated from the badgers examined. TheC. upsaliensisisolates were subjected to antimicrobial susceptibility testing against 8 antimicrobial agents. This revealed that most of the isolates from raccoon dogs were susceptible to the antimicrobial agents examined, whereas strains isolated from healthy dogs in Miyazaki prefecture, showed high rates of resistance. Virulence genes (flaA,cadF,ciaB,cdtA,cdtB, andcdtC) were present in theC. jejuniisolate from a raccoon dog, with the exception offlaB. By contrast, all these virulence genes examined were present in allC. upsaliensisstrains isolated from raccoon dogs and dogs. The genetic diversity of those isolates based on the nucleotide sequences of 7 housekeeping genes (adk, aspA, atpA, glnA, glyA, pgi, tkt) was compared with that ofC. upsaliensisstrains isolated from dogs and strains selected randomly from humans and dogs deposited in theCampylobacterMLST database. The major cluster of raccoon dog strains was separated from both human and dog strains by phylogenetic tree analysis. These results suggest that raccoon dogs are a reservoir ofC. upsaliensisand that isolates may represent a population different from that in humans and dogs. To our knowledge, this is the first study to have demonstrated a high prevalence ofC. upsaliensisin raccoon dogs.
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对布鲁塞尔日托中心乌普萨利弯曲杆菌爆发的调查：通过表型和基因型分型方法分析分离株之间的关系。
影响因子：
6.4
作者：
H. Goossens;B. Giesendorf;Peter Vandamme;L. Vlaes;C. Borre;A. Koeken;Wim Quint;W. Blomme;P. Hanicq;D. Koster;H. Hofstra;J. Butzler;J. V. D. Plas
通讯作者：
J. V. D. Plas
影响因子：
1.2
作者：
Aoyagi, T;Sato, Y;Wada, H
通讯作者：
Wada, H
影响因子：
2.6
作者：
Sandberg, M;Bergsjo, B;Kruse, H
通讯作者：
Kruse, H
影响因子：
2.2
作者：
Kauhala, Kaarina;Kowalczyk, Rafal
通讯作者：
Kowalczyk, Rafal
从狗中分离出无过氧化氢酶活性或过氧化氢酶活性较弱的耐热弯曲杆菌
影响因子：
2.6
作者：
K. Sandstedt;J. Ursing;M. Walder
通讯作者：
M. Walder