Role of vacA and cagA inHelicobacter pylori Inhibition of Mucin Synthesis in Gastric Mucous Cells
Role of vacA and cagA inHelicobacter pylori Inhibition of Mucin Synthesis in Gastric Mucous Cells
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vacA和cagA在幽门螺杆菌抑制胃粘液细胞粘蛋白合成中的作用
DOI：
10.1128/jcm.38.6.2215-2218.2000
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发表时间：
2000
影响因子：
9.4
通讯作者：
S. Wagner
中科院分区：
文献类型：
--
作者：
W. Beil;M. Enss;S. Müller;B. Obst;K. Sewing;S. Wagner
摘要本研究旨在探讨幽门螺杆菌对胃粘膜细胞功能的影响。幽门螺杆菌(Hp)(104~107CFU/孔)与产生粘蛋白的胃细胞系HM02孵育12和24 h，通过向细胞内掺入d-N-[乙酰-14C]氨基葡萄糖来测定粘蛋白的合成和分泌并释放高相对分子质量的糖蛋白。与≥10 5菌量(菌胞比=0.2 5)共培养12h和2 4h后，粘蛋白合成受到明显抑制。CagA阳性的细胞毒素产生菌(HP64、HP57和HP87)对细胞内粘蛋白合成的抑制作用明显强于cagA阳性的非细胞毒素产生菌(HP05、HP83和HP84)。不产生细胞毒素的agA阴性菌株(HP01、HP04和HP85)不影响细胞内粘蛋白的合成。结果表明，幽门螺杆菌直接损害胃粘液细胞的粘蛋白合成，细胞毒性cagA阳性菌株对粘蛋白合成的抑制作用更强。我们认为，cagA阳性的产生细胞毒素的菌株对粘蛋白合成的抑制作用可能是导致这些幽门螺杆菌菌株发生消化性溃疡风险增加的一个可能因素。
ABSTRACT The aim of this study was to investigate the effect ofHelicobacter pylori on the function of gastric mucous cells. H. pylori (104 to 107CFU/well) was incubated with the mucin-producing gastric cell line HM02 for 12 and 24 h. Mucin synthesis and secretion were determined by the incorporation ofd-N-[acetyl-14C]glucosamine into intracellular and released high-molecular-weight glycoproteins.cagA-positive, cytotoxin-producing and non-cytotoxin-producing H. pylori strains impaired the incorporation ofd-N-[acetyl-14C]glucosamine into intracellular glycoproteins. Significant inhibition of mucin synthesis was noted after 12 and 24 h of cocultivation with a bacterial load of ≥105 bacteria (bacterium/cell ratio = 0.25). The cagA-positive, cytotoxin-producing strains (HP64, HP57, and HP87) caused significantly stronger inhibition of intracellular mucin synthesis than the cagA-positive, non-cytotoxin-producing strains (HP05, HP83, and HP84). ThecagA-negative, non-cytotoxin-producing strains (HP01, HP04, and HP85) did not affect intracellular mucin synthesis. The results indicate that H. pylori directly impairs mucin synthesis in gastric mucous cells and that cytotoxic cagA-positive strains cause more profound inhibition of mucin synthesis. We suggest that the increased inhibitory effect of cagA-positive, cytotoxin-producing strains on mucin synthesis can be considered one possible factor responsible for the increased risk of developing peptic ulceration with these H. pylori strains.
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幽门螺杆菌的磷脂酶活性及其对铋盐的抑制作用。
影响因子：
3.1
作者：
Ottlecz,A;Romero,JJ;Hazell,SL;Graham,DY;Lichtenberger,LM
通讯作者：
Lichtenberger,LM
影响因子：
56.9
作者：
BOREN, T;FALK, P;NORMARK, S
通讯作者：
NORMARK, S
幽门弯曲杆菌降解粘蛋白并破坏胃粘膜完整性。
DOI：
10.1016/s0006-291x(87)80511-9
发表时间：
1987
影响因子：
3.1
作者：
Slomiany,BL;Bilski,J;Sarosiek,J;Murty,VL;Dworkin,B;VanHorn,K;Zielenski,J;Slomiany,A
通讯作者：
Slomiany,A
DOI：
10.1073/pnas.90.5.2035
发表时间：
1993-03-01
影响因子：
11.1
作者：
FALK, P;ROTH, KA;NORMARK, S
通讯作者：
NORMARK, S
影响因子：
3.5
作者：
Xiao Geng Chen;P. Correa;J. Offerhaus;Elsie Rodriguez;F. Janney;E. Hoffmann;James G. Fox;F. Hunter;S. Diavolitsis
通讯作者：
Xiao Geng Chen;P. Correa;J. Offerhaus;Elsie Rodriguez;F. Janney;E. Hoffmann;James G. Fox;F. Hunter;S. Diavolitsis