Surface enhanced Raman spectroscopy in microchip electrophoresis.
Surface enhanced Raman spectroscopy in microchip electrophoresis.
复制标题
微芯片电泳中的表面增强拉曼光谱
DOI：
10.1016/j.chroma.2018.02.014
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
D. Belder
中科院分区：
文献类型：
--
作者：
A. Tycova;R. F. Gerhardt;D. Belder
微芯片毛细管电泳联用表面增强拉曼光谱(MCE-SERS)结合了毛细管电泳高分离能力和获得振动指纹图谱用于化合物鉴定的能力。拉曼光谱是一种结构描述和无标记的检测方法，特别适合于化学分析，因为它是非破坏性的，可以识别分析物。然而，它的灵敏度很低，有时需要的采集时间远远长于典型的电泳分离峰宽。如果分析物由于表面增强效应而接近纳米结构的金属表面或胶体，则拉曼强度可以显著提高。提出了一种用于MCE-SERS在线耦合的新方法。所开发的玻璃微流控装置的关键部件是加药结构，该加药结构由两个侧通道组成，在分析物的电泳分离后对称地连接MCE通道。给药通道将银纳米颗粒(Ag-NPs)提供给分离的电泳区，这有助于对分离的化合物的SERS光谱进行即时记录。通过对90 范围内的罗丹明模型混合物的分析，对芯片的功能进行了评估，获得了相对标准偏差小于1.5%的迁移时间。该方法已成功应用于烤肉酱中食品添加剂核黄素的分析。
Coupling microchip capillary electrophoresis to surface enhanced Raman spectroscopy (MCE-SERS) combines the high separation power of capillary electrophoresis with the capability to obtain vibrational fingerprint spectra for compound identification. Raman spectroscopy is a structurally descriptive and label-free detection method which is particularly suited for chemical analysis because it is non-destructive and allows the identification of analytes. However, it suffers from poor sensitivity and sometimes even requires acquisition times far longer than the typical peak width of electrophoretic separations. The Raman intensity can be drastically improved if the analyte is brought into close proximity to nanostructured metal surfaces or colloids due to the surface enhancement effect. This paper presents a novel approach in the field of MCE-SERS on-line coupling. The key element of the developed glass microfluidic device is a dosing structure which consists of two side channels joining the MCE channel symmetrically after the electrophoretic separation of the analytes. The dosing channel supplies silver nanoparticles (Ag-NPs), to the separated electrophoretic zones which facilitates an on-the-fly recording of SERS-spectra of the separated compounds. The functionality of the MCE-SERS chip was evaluated by the analysis of a rhodamine model mixture within 90 s achieving RSD of migration times below 1.5%. The approach was successfully applied for the analysis of the food additive riboflavin in a barbecue sauce.
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影响因子：
7.4
作者：
Choi, Namhyun;Lee, Jiyoung;Choo, Jaebum
通讯作者：
Choo, Jaebum
影响因子：
4.3
作者：
Haelvin, Kristel;Paalme, Toomas;Nisamedtinov, Ildar
通讯作者：
Nisamedtinov, Ildar
DOI：
10.1016/j.saa.2011.09.043
发表时间：
2012-01-01
影响因子：
4.4
作者：
Liu, Fangfang;Gu, Huaimin;Cai, Tiantian
通讯作者：
Cai, Tiantian
用于微流控柱阵列分离芯片的表面增强拉曼光谱。
DOI：
10.1039/c2an16239c
发表时间：
2012
期刊：
The Analyst
影响因子：
--
作者：
L. C. Taylor;Teresa B Kirchner;N. Lavrik;M. Sepaniak
通讯作者：
M. Sepaniak
影响因子：
5.7
作者：
D. Geissler;Josef J. Heiland;Carsten Lotter;D. Belder
通讯作者：
D. Geissler;Josef J. Heiland;Carsten Lotter;D. Belder