A Retinol Isotope Dilution Equation Predicts Both Group and Individual Total Body Vitamin A Stores in Adults Based on Data from an Early Postdosing Blood Sample.
A Retinol Isotope Dilution Equation Predicts Both Group and Individual Total Body Vitamin A Stores in Adults Based on Data from an Early Postdosing Blood Sample.
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DOI：
10.3945/jn.116.233676
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发表时间：
2016-10
期刊：
影响因子：
--
通讯作者：
Lietz G
中科院分区：
文献类型：
--
作者：
Green MH;Ford JL;Green JB;Berry P;Boddy AV;Oxley A;Lietz G
背景：视黄醇同位素稀释法（RID）用于测定口服维生素A稳定同位素后的维生素A全身储存量（TBS）。Olson小组在1989年提出的普遍接受的预测方程（Furr等，Am J Clin Nutr 1989;49：713-6）包括与剂量吸收和保留、与储存相比血浆中的同位素平衡、混合期间的催化剂以及测量血浆同位素富集的最佳时间有关的因素。目的：目的是1）开发一个修改的RID方程，并确定一个更早的采样时间预测TBS和2）以提高预测个人以及群体。研究方法：为了开发改良的RID方程，我们使用了http://www.WinSAAM.org32名既往研究的欧洲血统健康年轻成人的血浆[13 C10]视黄醇动力学数据的基于模型的房室分析结果[模拟、分析和建模软件（WinSAAM版本3.0.8; www.example.com）]，这些人摄入了中等维生素A，摄入了2.95 μmol [13 C10]醋酸视黄酯。结果如下：我们检查了与吸收/保留（Fa）和同位素平衡（S）相关的预测方程中因子的时间依赖性，并确定给药后4或5 d是最佳采样时间。通过公式TBS = Fa x S x（1/SAp）计算的TBS（其中SAp是血浆视黄醇比活性（剂量分数/μmol））与模型预测的TBS高度相关（4 d和5 d分别为r = 0.95和0.96; P < 0.001）;个体预测值也高度相关（Rs = 0.94和0.94; P < 0.001）。结论：TBS = 0.5 ×（1/SAp）方程可准确地预测32名健康青年及其个体的维生素A TBS值。
Background: Retinol isotope dilution (RID) is used to determine vitamin A total body stores (TBS) after an oral dose of a vitamin A stable isotope. The generally accepted prediction equation proposed by Olson’s group in 1989 (Furr et al. Am J Clin Nutr 1989;49:713–6) includes factors related to dose absorption and retention, isotope equilibration in plasma compared with stores, catabolism during the mixing period, and the optimal time for measuring plasma isotope enrichment. Objectives: The objectives were 1) to develop a modified RID equation and identify an earlier sampling time for predicting TBS and 2) to improve prediction in individuals as well as groups. Methods: To develop a modified RID equation, we used results of model-based compartmental analysis [the Simulation, Analysis and Modeling software (WinSAAM version 3.0.8; http://www.WinSAAM.org)] of plasma [13C10]retinol kinetic data from 32 previously studied, healthy young adults of European ancestry who had moderate vitamin A intakes and who ingested 2.95 μmol [13C10]retinyl acetate. Results: We examined the time dependence of factors in the prediction equation related to absorption/retention (Fa) and isotope equilibration (S) and determined that 4 or 5 d postdosing was the optimal sampling time. TBS calculated by the equation TBS = Fa x S x (1/SAp), where SAp is plasma retinol specific activity (fraction of dose/μmol), were highly correlated with model-predicted TBS (r = 0.95 and 0.96 for 4 and 5 d, respectively; P < 0.001); predictions for individuals were also highly correlated (Rs = 0.94 and 0.94; P < 0.001). Conclusion: The equation TBS ≈ 0.5 × (1/SAp) accurately predicted vitamin A TBS in this group of 32 healthy young adults and its individual members with the use of data from 1 blood sample taken 4 d after isotope administration.
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影响因子：
4.2
作者：
GREEN, MH;GREEN, JB
通讯作者：
GREEN, JB
影响因子：
--
作者：
Green, M H;Green, J B
通讯作者：
Green, J B
DOI：
10.3945/jn.116.233486
发表时间：
2016-10
期刊：
The Journal of nutrition
影响因子：
--
作者：
Green MH;Ford JL;Oxley A;Green JB;Park H;Berry P;Boddy AV;Lietz G
通讯作者：
Lietz G
影响因子：
7.1
作者：
Tang, GW;Qin, J;Russell, RM
通讯作者：
Russell, RM
影响因子：
7.1
作者：
Haskell, MJ;Handelman, GJ;Brown, KH
通讯作者：
Brown, KH