Plasma Retinol Kinetics and β-Carotene Bioefficacy Are Quantified by Model-Based Compartmental Analysis in Healthy Young Adults with Low Vitamin A Stores.
Plasma Retinol Kinetics and β-Carotene Bioefficacy Are Quantified by Model-Based Compartmental Analysis in Healthy Young Adults with Low Vitamin A Stores.
复制标题
DOI：
10.3945/jn.116.233486
复制
发表时间：
2016-10
期刊：
影响因子：
--
通讯作者：
Lietz G
中科院分区：
文献类型：
--
作者：
Green MH;Ford JL;Oxley A;Green JB;Park H;Berry P;Boddy AV;Lietz G
背景：对给予标记视黄醇后血浆视黄醇动力学数据进行的基于模型的分室分析提供了有关全身维生素A代谢的独特信息。如果联合使用标记的β-胡萝卜素，也可以估计其相对于视黄醇参考剂量的生物有效性。目的：模拟给予标记的预制维生素A和维生素A原β-胡萝卜素后的血浆视黄醇动力学，并确定相对的β-胡萝卜素的生物效应。方法：用模拟、分析和建模软件(WinSAAM3.0.8版；http://www.WinSAAM.org))对30名欧洲血统的健康青年[男性13人，女性17人；平均±SD年龄：24.5±4.2岁；平均±SD体重：65.2±10 kg；平均±SD体重：65.2±10 kg；平均±标准差体重指数(单位：公斤/平方米)：22.5±1.9]，维生素A摄入量适中。结果：6组分模型最符合数据，包括维生素A初始代谢、血浆视黄醇和血管外维生素A储存的分区。处理速度为6.7±3.1μm ol/d，分解率为6.0%±2.3%/d，维生素A贮存量为12 3±71μm ol。β-胡萝卜素的相对生物效应，基于WinSAAM计算的剂量曲线分数下的面积比，平均为13.5%±6.02%(视黄醇活性当量=7.7：1.0μg)。相对β-胡萝卜素生物效应的个体间变异较大(CV：44%)。结论：这些年轻人体内的维生素A动力学与之前使用维生素A储备量较高的老年人的数据建立的模型基本上是相同的；参数值的差异反映了维生素A状态的差异。估计的β-胡萝卜素生物功效相对较低，但与之前报道的通过图形方法获得的估计值相似。这项试验在英国临床研究网络注册为UKCRN 7413。
Background: Model-based compartmental analysis of data on plasma retinol kinetics after administration of labeled retinol provides unique information about whole-body vitamin A metabolism. If labeled β-carotene is coadministered, its bioefficacy relative to the retinol reference dose can also be estimated. Objectives: The objectives were to model plasma retinol kinetics after administration of labeled preformed vitamin A and provitamin A β-carotene and to determine relative β-carotene bioefficacy. Methods: We used the Simulation, Analysis and Modeling software (WinSAAM version 3.0.8; http://www.WinSAAM.org) to analyze previously collected data on plasma [13C10]- and [13C5]retinol kinetics for 14 d after oral administration of 1 mg [13C10]retinyl acetate and 2 mg [13C10]β-carotene in oil to 30 healthy young adults of European ancestry [13 men, 17 women; mean ± SD age: 24.5 ± 4.2 y; mean ± SD body weight: 65.2 ± 10 kg; mean ± SD body mass index (in kg/m2): 22.5 ± 1.9] with moderate vitamin A intakes. Results: A 6-component model provided the best fit to the data, including compartments for initial metabolism of vitamin A, plasma retinol, and extravascular vitamin A storage. The disposal rate was 6.7 ± 3.1 μmol/d, fractional catabolic rate was 6.0% ± 2.3%/d, and vitamin A stores were 123 ± 71 μmol. Relative β-carotene bioefficacy, based on the ratio of the areas under the fraction of dose curves calculated by WinSAAM, averaged 13.5% ± 6.02% (retinol activity equivalents = 7.7:1.0 μg). Interindividual variation in relative β-carotene bioefficacy was high (CV: 44%). Conclusions: Vitamin A kinetics in these young adults were best described by essentially the same model that had been previously developed by using data for older adults with higher vitamin A stores; differences in parameter values reflected differences in vitamin A status. Estimated β-carotene bioefficacy was relatively low but similar to previously reported estimates obtained by graphical methods. This trial was registered at the UK Clinical Research Network as UKCRN 7413.
登录
查看更多内容
影响因子：
3.6
作者：
Wang, ZX;Yin, S;Tang, GW
通讯作者：
Tang, GW
影响因子：
4.2
作者：
Kelley, SK;Green, MH
通讯作者：
Green, MH
影响因子：
7.1
作者：
Tang, Guangwen
通讯作者：
Tang, Guangwen
影响因子：
7.1
作者：
Wang, Jie;Wang, Yin;Yin, Shian
通讯作者：
Yin, Shian
DOI：
10.3945/jn.116.233676
发表时间：
2016-10
期刊：
The Journal of nutrition
影响因子：
--
作者：
Green MH;Ford JL;Green JB;Berry P;Boddy AV;Oxley A;Lietz G
通讯作者：
Lietz G