Iridovirus and microsporidian linked to honey bee colony decline.
Iridovirus and microsporidian linked to honey bee colony decline.
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DOI：
10.1371/journal.pone.0013181
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发表时间：
2010-10-06
期刊：
影响因子：
3.7
通讯作者：
Cramer RA Jr
中科院分区：
文献类型：
--
作者：
Bromenshenk JJ;Henderson CB;Wick CH;Stanford MF;Zulich AW;Jabbour RE;Deshpande SV;McCubbin PE;Seccomb RA;Welch PM;Williams T;Firth DR;Skowronski E;Lehmann MM;Bilimoria SL;Gress J;Wanner KW;Cramer RA Jr
2010年，蜂群衰竭失调症（Colony Collapse Disorder，CCD）再次摧毁了美国的蜜蜂群，这表明这个问题既没有减少，也没有得到解决。许多CCD研究，使用敏感的基因组为基础的方法，已经发现了小RNA蜜蜂病毒和微孢子虫，蜜蜂微孢子虫和N。在健康和崩溃的蜂群中，没有任何单一的病原体与蜜蜂的损失密切相关。我们使用基于质谱的蛋白质组学（MSP）来识别和量化健康和崩溃的蜂群中的数千种蛋白质。MSP在北美蜜蜂中发现了两种未报道的RNA病毒，Varroa destructor-1病毒和Kakugo病毒，并鉴定了与CCD菌落相关的无脊椎动物虹彩病毒（IIV）（虹彩病毒科）。IIV的患病率显着歧视强，失败，崩溃的殖民地。此外，在失败的殖民地蜜蜂不仅包含IIV，但也微粒子。这些微生物的共同出现始终标志着CCD（1）2006-2007年在美国各地的商业养蜂场采样的蜜蜂，（2）2008年在观察蜂巢群中随着疾病进展而连续采样的蜜蜂，以及（3）2009年在佛罗里达复发CCD的蜜蜂。在没有CCD历史的殖民地样本中没有观察到病原体配对，即来自澳大利亚的蜜蜂和蒙大拿州的大型非迁移养蜂企业。用IIV 6型菌株和Nosema cerebral进行的实验室笼试验证实，这两种病原体的共同感染对蜜蜂的致命性比单独感染任何一种病原体都高。这些发现暗示了IIV和微孢子虫的共同感染与蜜蜂群体的下降，使人们相信早期的研究指出IIV与微孢子虫和螨相互作用，可能是美国，欧洲和亚洲蜜蜂损失的原因。接下来，我们需要描述我们检测到的IIV和微孢子虫的特征，并制定管理措施以减少蜜蜂损失。
In 2010 Colony Collapse Disorder (CCD), again devastated honey bee colonies in the USA, indicating that the problem is neither diminishing nor has it been resolved. Many CCD investigations, using sensitive genome-based methods, have found small RNA bee viruses and the microsporidia, Nosema apis and N. ceranae in healthy and collapsing colonies alike with no single pathogen firmly linked to honey bee losses. We used Mass spectrometry-based proteomics (MSP) to identify and quantify thousands of proteins from healthy and collapsing bee colonies. MSP revealed two unreported RNA viruses in North American honey bees, Varroa destructor-1 virus and Kakugo virus, and identified an invertebrate iridescent virus (IIV) (Iridoviridae) associated with CCD colonies. Prevalence of IIV significantly discriminated among strong, failing, and collapsed colonies. In addition, bees in failing colonies contained not only IIV, but also Nosema. Co-occurrence of these microbes consistently marked CCD in (1) bees from commercial apiaries sampled across the U.S. in 2006–2007, (2) bees sequentially sampled as the disorder progressed in an observation hive colony in 2008, and (3) bees from a recurrence of CCD in Florida in 2009. The pathogen pairing was not observed in samples from colonies with no history of CCD, namely bees from Australia and a large, non-migratory beekeeping business in Montana. Laboratory cage trials with a strain of IIV type 6 and Nosema ceranae confirmed that co-infection with these two pathogens was more lethal to bees than either pathogen alone. These findings implicate co-infection by IIV and Nosema with honey bee colony decline, giving credence to older research pointing to IIV, interacting with Nosema and mites, as probable cause of bee losses in the USA, Europe, and Asia. We next need to characterize the IIV and Nosema that we detected and develop management practices to reduce honey bee losses.
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影响因子：
3.4
作者：
Chen, Yanping;Evans, Jay;Feldlaufer, Mark
通讯作者：
Feldlaufer, Mark
影响因子：
4.6
作者：
COLE, A;MORRIS, TJ
通讯作者：
MORRIS, TJ
影响因子：
3.7
作者：
Chitnis NS;D'Costa SM;Paul ER;Bilimoria SL
通讯作者：
Bilimoria SL
DOI：
10.1073/pnas.0906970106
发表时间：
2009-09-01
影响因子：
11.1
作者：
Johnson, Reed M.;Evans, Jay D.;Berenbaum, May R.
通讯作者：
Berenbaum, May R.
影响因子：
2.7
作者：
D'Costa, SM;Yao, HJ;Bilimoria, SL
通讯作者：
Bilimoria, SL