Brown adipogenic potential of brown adipocytes and peri-renal adipocytes from human embryo.
Brown adipogenic potential of brown adipocytes and peri-renal adipocytes from human embryo.
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来自人类胚胎的棕色脂肪细胞和肾周脂肪细胞的棕色脂肪形成潜力。
DOI：
10.1038/srep39193
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发表时间：
2016-12-16
影响因子：
4.6
通讯作者：
Feng YM
中科院分区：
文献类型：
--
作者：
Wu NN;Zhang CH;Lee HJ;Ma Y;Wang X;Ma XJ;Ma W;Zhao D;Feng YM
棕色脂肪细胞（BAC）和米色细胞都对代谢紊乱的治疗具有潜在疗效。不幸的是，成年人中这些细胞的数量和活性有限。尽管已在儿童和成人的肾周脂肪组织中发现了BAC标志物的表达，但缺乏功能评估。此外，完全不清楚人类胚胎中是否存在脂肪祖细胞，以及在发育过程中是否能够原位产生BAC。因此，从人类胚胎中解剖出肩胛间和肾周区域的脂肪组织，并从一名成年人获取皮下白色脂肪组织（sWAT）。在体外进行分化后，在所有这些脂肪组织中都检测到存在脂肪细胞祖细胞。当受到脂肪生成刺激时，肩胛间和肾周区域分化的脂肪细胞表现出相似的特征：（1）诱导产生BAC和米色细胞标志物的表达，包括解偶联蛋白1（UCP1）和含PR结构域16（PRDM16），且线粒体拷贝数相当；（2）通过RNA - Seq分析得到相似的基因表达模式；（3）通过呼吸测定法检测到相似的最大耗氧率。然而，刺激sWAT中的脂肪细胞祖细胞既不会诱导BAC和米色细胞标志物的表达，也不会引起耗氧量的任何变化。总之，人类胚胎中的肾周脂肪细胞祖细胞具有产生BAC的棕色化潜能。
Both brown adipocytes (BAC) and beige cells hold therapeutic potential for the treatment of metabolic disorders. Unfortunately, the amount and activity of these cells are limited in adults. Although BAC marker expression has been shown in peri-renal adipose tissues in children and adults, functional assessment is lacking. Furthermore, it is entirely unknown whether adipose progenitors are present in human embryo and able to give rise to BAC in situ during evolution. Therefore, adipose tissues in the interscapular and peri-renal regions were dissected from human embryo and subcutaneous white adipose tissues (sWAT) were obtained from an adult. After subjected to differentiation in vitro, adipocyte progenitors were detected present in all these adipose tissues. When stimulated for adipogenesis, differentiated adipocytes in the intercapular and peri-renal regions showed similar features: (1) induced BAC and beige cell marker expression including UCP1 and PRDM16 and comparable mitochondrion copy number; (2) similar gene expression patterns by RNA-Seq analysis; and (3) similar maximal oxygen consumption rates examined by respirometry. Nevertheless, stimulation of adipocyte progenitors in sWAT induces neither BAC and beige cell marker expression nor any change of oxygen consumption. In conclusion, peri-renal adipocyte progenitors in human embryo hold browning potential for BAC production.
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影响因子：
3.7
作者：
Nagano G;Ohno H;Oki K;Kobuke K;Shiwa T;Yoneda M;Kohno N
通讯作者：
Kohno N
影响因子：
21.3
作者：
通讯作者：
--
高血压患者皮下及肾周脂肪组织中UCP1表达的测定
影响因子：
3.7
作者：
Li, Xueqin;Liu, Juan;Ding, Guoxian
通讯作者：
Ding, Guoxian
影响因子：
64.5
作者：
Cohen P;Levy JD;Zhang Y;Frontini A;Kolodin DP;Svensson KJ;Lo JC;Zeng X;Ye L;Khandekar MJ;Wu J;Gunawardana SC;Banks AS;Camporez JP;Jurczak MJ;Kajimura S;Piston DW;Mathis D;Cinti S;Shulman GI;Seale P;Spiegelman BM
通讯作者：
Spiegelman BM
影响因子：
4.8
作者：
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通讯作者：
Nedergaard, Jan