Activation of classical brown adipocytes in the adult human perirenal depot is highly correlated with PRDM16-EHMT1 complex expression.
Activation of classical brown adipocytes in the adult human perirenal depot is highly correlated with PRDM16-EHMT1 complex expression.
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DOI：
10.1371/journal.pone.0122584
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Kohno N
中科院分区：
文献类型：
--
作者：
Nagano G;Ohno H;Oki K;Kobuke K;Shiwa T;Yoneda M;Kohno N
棕色脂肪产生热量以防止寒冷和肥胖。肾上腺素能刺激激活棕色脂肪细胞的产热程序。尽管人们认为成年人棕色脂肪组织的生物活性非常低，但一些使用正电子发射断层扫描-计算机断层扫描（PET-CT）的研究已经检测到成年人在冷暴露下的生物活性棕色脂肪组织。在这项研究中，我们收集了从患有嗜铬细胞瘤（PHEO）或无功能肾上腺肿瘤（NF）的成年患者的肾周区域获得的脂肪组织。我们证明，成年 PHEO 患者的肾周棕色脂肪细胞被激活。这些细胞具有经典棕色脂肪的分子特征，而不是米色/英国脂肪的分子特征。棕色脂肪组织标志物，如解偶联蛋白 1 (UCP1) 和细胞死亡诱导 DFFA 样效应子 A (CIDEA) 的表达与 PRD1-BF-1-RIZ1 同源结构域蛋白 16 (PRDM16)——常染色组蛋白赖氨酸 N-甲基转移酶 1 (EHMT1) 复合物（棕色脂肪发育的关键转录开关）的数量高度相关。这些结果为人类棕色脂肪细胞的重建及其针对肥胖及其并发症（如 2 型糖尿病）的治疗应用提供了新的见解。
Brown fat generates heat to protect against cold and obesity. Adrenergic stimulation activates the thermogenic program of brown adipocytes. Although the bioactivity of brown adipose tissue in adult humans had been assumed to very low, several studies using positron emission tomography–computed tomography (PET–CT) have detected bioactive brown adipose tissue in adult humans under cold exposure. In this study, we collected adipose tissues obtained from the perirenal regions of adult patients with pheochromocytoma (PHEO) or non-functioning adrenal tumors (NF). We demonstrated that perirenal brown adipocytes were activated in adult patients with PHEO. These cells had the molecular characteristics of classical brown fat rather than those of beige/brite fat. Expression of brown adipose tissue markers such as uncoupling protein 1 (UCP1) and cell death-inducing DFFA-like effector A (CIDEA) was highly correlated with the amounts of PRD1-BF-1-RIZ1 homologous domain-containing protein-16 (PRDM16) – euchromatic histone-lysine N-methyltransferase 1 (EHMT1) complex, the key transcriptional switch for brown fat development. These results provide novel insights into the reconstruction of human brown adipocytes and their therapeutic application against obesity and its complications such as type 2 diabetes.
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影响因子：
7.7
作者：
Saito M;Okamatsu-Ogura Y;Matsushita M;Watanabe K;Yoneshiro T;Nio-Kobayashi J;Iwanaga T;Miyagawa M;Kameya T;Nakada K;Kawai Y;Tsujisaki M
通讯作者：
Tsujisaki M
影响因子：
29
作者：
Ohno H;Shinoda K;Spiegelman BM;Kajimura S
通讯作者：
Kajimura S
影响因子：
82.9
作者：
Lidell, Martin E.;Betz, Matthias J.;Enerback, Sven
通讯作者：
Enerback, Sven
影响因子：
64.5
作者：
Cohen P;Levy JD;Zhang Y;Frontini A;Kolodin DP;Svensson KJ;Lo JC;Zeng X;Ye L;Khandekar MJ;Wu J;Gunawardana SC;Banks AS;Camporez JP;Jurczak MJ;Kajimura S;Piston DW;Mathis D;Cinti S;Shulman GI;Seale P;Spiegelman BM
通讯作者：
Spiegelman BM
影响因子：
29
作者：
Cypess AM;Weiner LS;Roberts-Toler C;Franquet Elía E;Kessler SH;Kahn PA;English J;Chatman K;Trauger SA;Doria A;Kolodny GM
通讯作者：
Kolodny GM