An ABA-mimicking ligand that reduces water loss and promotes drought resistance in plants.
An ABA-mimicking ligand that reduces water loss and promotes drought resistance in plants.
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一种模拟 ABA 的配体，可减少水分流失并促进植物的抗旱性
作者：
脱落酸（阿坝）是植物抵抗干旱和其他非生物胁迫的最重要的激素。阿坝直接与阿坝受体的PYR/PYL家族结合，导致2C型磷酸酶（PP 2C）的抑制和下游阿坝信号传导的激活。据设想，阿坝信号的小分子干预可以帮助植物克服非生物胁迫，如干旱，寒冷和土壤盐碱。但是阿坝本身的化学性质不稳定和被植物酶快速催化的特性限制了它的实际应用。在这里，我们报告的小分子阿坝模拟物（AM 1），作为一个有效的激活剂的阿坝受体家族的多个成员的鉴定。在拟南芥中，AM 1激活的基因网络与阿坝诱导的基因网络高度相似。AM 1处理抑制种子萌发，防止叶片失水，提高抗旱性。我们解决了AM 1与PYL 2阿坝受体和HAB 1 PP 2C复合的晶体结构，这表明AM 1介导了一个门-锁-锁相互作用网络，这是ABA结合受体/PP 2C复合物中保守的结构特征。总之，这些结果表明，单个小分子阿坝模拟物可以激活多种阿坝受体，并保护植物免受水分流失和干旱胁迫。此外，AM 1复合物晶体结构为设计下一代ABA模拟小分子提供了结构基础。
Abscisic acid (ABA) is the most important hormone for plants to resist drought and other abiotic stresses. ABA binds directly to the PYR/PYL family of ABA receptors, resulting in inhibition of type 2C phosphatases (PP2C) and activation of downstream ABA signaling. It is envisioned that intervention of ABA signaling by small molecules could help plants to overcome abiotic stresses such as drought, cold and soil salinity. However, chemical instability and rapid catabolism by plant enzymes limit the practical application of ABA itself. Here we report the identification of a small molecule ABA mimic (AM1) that acts as a potent activator of multiple members of the family of ABA receptors. In Arabidopsis, AM1 activates a gene network that is highly similar to that induced by ABA. Treatments with AM1 inhibit seed germination, prevent leaf water loss, and promote drought resistance. We solved the crystal structure of AM1 in complex with the PYL2 ABA receptor and the HAB1 PP2C, which revealed that AM1 mediates a gate-latch-lock interacting network, a structural feature that is conserved in the ABA-bound receptor/PP2C complex. Together, these results demonstrate that a single small molecule ABA mimic can activate multiple ABA receptors and protect plants from water loss and drought stress. Moreover, the AM1 complex crystal structure provides a structural basis for designing the next generation of ABA-mimicking small molecules.
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DOI：
10.1126/science.1173041
发表时间：
2009-05-22
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Park SY;Fung P;Nishimura N;Jensen DR;Fujii H;Zhao Y;Lumba S;Santiago J;Rodrigues A;Chow TF;Alfred SE;Bonetta D;Finkelstein R;Provart NJ;Desveaux D;Rodriguez PL;McCourt P;Zhu JK;Schroeder JI;Volkman BF;Cutler SR
通讯作者：
Cutler SR
脱甲酸受体的激素信号传导的栅极锁锁机制。
影响因子：
64.8
作者：
通讯作者：
--
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
影响因子：
16.8
作者：
Yin, Ping;Fan, He;Yan, Nieng
通讯作者：
Yan, Nieng
DOI：
10.1126/science.1215106
发表时间：
2012-01-06
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Soon FF;Ng LM;Zhou XE;West GM;Kovach A;Tan MH;Suino-Powell KM;He Y;Xu Y;Chalmers MJ;Brunzelle JS;Zhang H;Yang H;Jiang H;Li J;Yong EL;Cutler S;Zhu JK;Griffin PR;Melcher K;Xu HE
通讯作者：
Xu HE