A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors.
A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors.
复制标题
脱甲酸受体的激素信号传导的栅极锁锁机制。
作者：
脱落酸（阿坝）是一种普遍存在的激素，调节植物的生长，发育和对环境胁迫的反应。它的作用是由START蛋白的PYR/PYL/RCAR家族介导的，但仍不清楚这些受体如何结合阿坝，以及激素结合如何导致下游2C型蛋白磷酸酶（PP 2C）效应物的抑制。在这里，我们报告的晶体结构的载脂蛋白和ABA结合的受体，以及三元PYL 2-ABA-PP 2C复合物。载脂蛋白受体含有一个开放的配体结合口袋，其两侧是一个门，该门通过两个高度保守的β环中的构象变化响应于阿坝而关闭，这两个高度保守的β环充当门和闩锁。此外，ABA诱导的门关闭产生了一个表面，使受体对接到和竞争性抑制PP 2C活性位点。PP 2C中的保守色氨酸直接插入门和闩锁之间，其功能是进一步将受体锁定在闭合构象中。总之，我们的研究结果确定了一个保守的门闩锁机制的阿坝信号。
Abscisic acid (ABA) is a ubiquitous hormone that regulates plant growth, development, and responses to environmental stresses. Its action is mediated by the PYR/PYL/RCAR family of START proteins, but it remains unclear how these receptors bind ABA and in turn, how hormone binding leads to inhibition of the downstream type 2C protein phosphatase (PP2C) effectors. Here we report crystal structures of apo and ABA-bound receptors as well as a ternary PYL2-ABA-PP2C complex. The apo receptors contain an open ligand-binding pocket flanked by a gate that closes in response to ABA via conformational changes in two highly conserved β-loops that serve as a gate and latch. Moreover, ABA-induced closure of the gate creates a surface that enables the receptor to dock into and competitively inhibit the PP2C active site. A conserved tryptophan in the PP2C inserts directly between the gate and latch, which functions to further lock the receptor in a closed conformation. Together, our results identify a conserved gate-latch-lock mechanism underlying ABA signaling.
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影响因子：
11.4
作者：
Chinnusamy V;Gong Z;Zhu JK
通讯作者：
Zhu JK
影响因子：
12.3
作者：
Schrick K;Nguyen D;Karlowski WM;Mayer KF
通讯作者：
Mayer KF
DOI：
10.1126/science.1173041
发表时间：
2009-05-22
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Park SY;Fung P;Nishimura N;Jensen DR;Fujii H;Zhao Y;Lumba S;Santiago J;Rodrigues A;Chow TF;Alfred SE;Bonetta D;Finkelstein R;Provart NJ;Desveaux D;Rodriguez PL;McCourt P;Zhu JK;Schroeder JI;Volkman BF;Cutler SR
通讯作者：
Cutler SR
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
DOI：
10.1073/pnas.0505667103
发表时间：
2006-02-07
影响因子：
11.1
作者：
Furihata, T;Maruyama, K;Yamaguchi-Shinozaki, K
通讯作者：
Yamaguchi-Shinozaki, K