Biocontrol of strawberry gray mold caused by Botrytis cinerea with the termite associated Streptomyces sp. sdu1201 and actinomycin D.
Biocontrol of strawberry gray mold caused by Botrytis cinerea with the termite associated Streptomyces sp. sdu1201 and actinomycin D.
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DOI：
10.3389/fmicb.2022.1051730
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发表时间：
2022
影响因子：
5.2
通讯作者：
Li, Ruijuan
中科院分区：
文献类型：
--
作者：
Yong, Daojing;Li, Yue;Gong, Kai;Yu, Yingying;Zhao, Shuai;Duan, Qiong;Ren, Cailing;Li, Aiying;Fu, Jun;Ni, Jinfeng;Zhang, Youming;Li, Ruijuan
由灰霉病菌引起的草莓灰霉病是草莓采前和采后最为严重的病害之一。虽然杀菌剂是控制该病的有效方法，但它们也会造成严重的“3R”问题(抗药性、卷土重来和残留)。本研究中，链霉菌属(Streptomyces sp.)从食用菌白蚁Odontotermes formosanus的后肠中分离到的SDU1201菌株具有明显的抗真菌活性。从链霉菌中分离得到4个化合物(1-4)。SDU1201，经HRMS和一维核磁共振数据进一步鉴定为放线菌素。其中，放线菌素D对草莓灰霉病的抑制作用最强，EC50值为7.6 5μg m L−1。对草莓灰霉病的防治效果也进行了体外果实和叶片的测定，3d时对叶片的防效为78.77%，并能抑制芽管的极化生长。因此，链霉菌(Streptomyces sp.)SDU1201和放线菌素D对灰霉菌有很大的生防潜力。
Strawberry gray mold caused by Botrytis cinerea is one of the most severe diseases in pre- and post-harvest periods. Although fungicides have been an effective way to control this disease, they can cause serious “3R” problems (Resistance, Resurgence and Residue). In this study, Streptomyces sp. sdu1201 isolated from the hindgut of the fungus-growing termite Odontotermes formosanus revealed significant antifungal activity against B. cinerea. Four compounds (1–4) were isolated from Streptomyces sp. sdu1201 and further identified as actinomycins by the HRMS and 1D NMR data. Among them, actinomycin D had the strongest inhibitory activity against B. cinerea with the EC50 value of 7.65 μg mL−1. The control effect of actinomycin D on strawberry gray mold was also tested on fruits and leaves in vitro, and its control efficiency on leaves was 78.77% at 3 d. Moreover, actinomycin D can also inhibit the polarized growth of germ tubes of B. cinerea. Therefore, Streptomyces sp. sdu1201 and actinomycin D have great potential to gray mold as biocontrol agents.
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DOI：
10.3390/plants10122737
发表时间：
2021-12-12
期刊：
Plants (Basel, Switzerland)
影响因子：
--
作者：
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通讯作者：
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影响因子：
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Stenberg, Johan A.
影响因子：
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作者：
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通讯作者：
Zeeck, Axel
影响因子：
5
作者：
Chen, Caixia;Song, Fuhang;Zhang, Lixin
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Zhang, Lixin
影响因子：
4
作者：
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