Two wheat glutathione peroxidase genes whose products are located in chloroplasts improve salt and H2O2 tolerances in Arabidopsis.
Two wheat glutathione peroxidase genes whose products are located in chloroplasts improve salt and H2O2 tolerances in Arabidopsis.
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两个小麦谷胱甘肽过氧化物酶基因的产物位于叶绿体中，可提高拟南芥对盐和 H2O2 的耐受性
DOI：
10.1371/journal.pone.0073989
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Ma YZ
中科院分区：
文献类型：
--
作者：
Zhai CZ;Zhao L;Yin LJ;Chen M;Wang QY;Li LC;Xu ZS;Ma YZ
Oxidative stress caused by accumulation of reactive oxygen species (ROS) is capable of damaging effects on numerous cellular components. Glutathione peroxidases (GPXs, EC 1.11.1.9) are key enzymes of the antioxidant network in plants. In this study, W69 and W106, two putative GPX genes, were obtained by de novo transcriptome sequencing of salt-treated wheat (Triticum aestivum) seedlings. The purified His-tag fusion proteins of W69 and W106 reduced H2O2 and t-butyl hydroperoxide (t-BHP) using glutathione (GSH) or thioredoxin (Trx) as an electron donor in vitro, showing their peroxidase activity toward H2O2 and toxic organic hydroperoxide. GFP fluorescence assays revealed that W69 and W106 are localized in chloroplasts. Quantitative real-time PCR (Q-RT-PCR) analysis showed that two GPXs were differentially responsive to salt, drought, H2O2, or ABA. Isolation of the W69 and W106 promoters revealed some cis-acting elements responding to abiotic stresses. Overexpression of W69 and W106 conferred strong tolerance to salt, H2O2, and ABA treatment in Arabidopsis. Moreover, the expression levels of key regulator genes (SOS1, RbohD and ABI1/ABI2) involved in salt, H2O2 and ABA signaling were altered in the transgenic plants. These findings suggest that W69 and W106 not only act as scavengers of H2O2 in controlling abiotic stress responses, but also play important roles in salt and ABA signaling.
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影响因子：
3.4
作者：
Bae YA;Cai GB;Kim SH;Zo YG;Kong Y
通讯作者：
Kong Y
DOI：
10.1016/j.bbagen.2005.04.018
发表时间：
2005-06-20
影响因子：
3
作者：
Herbette, S;Le Menn, A;Roeckel-Drevet, P
通讯作者：
Roeckel-Drevet, P
DOI：
10.1007/s11626-011-9438-z
发表时间：
2011-09-01
影响因子：
2.1
作者：
Baek, In-Jeoung;Jung, Ki Youn;Nam, Sang-Yoon
通讯作者：
Nam, Sang-Yoon
影响因子：
4.9
作者：
Faltin, Zehava;Holland, Doron;Perl, Avihai
通讯作者：
Perl, Avihai
影响因子：
3.5
作者：
Churin, Y;Schilling, S;Börner, T
通讯作者：
Börner, T