Modular evolution of glutathione peroxidase genes in association with different biochemical properties of their encoded proteins in invertebrate animals.
Modular evolution of glutathione peroxidase genes in association with different biochemical properties of their encoded proteins in invertebrate animals.
复制标题
DOI：
10.1186/1471-2148-9-72
复制
发表时间：
2009-04-06
影响因子：
3.4
通讯作者：
Kong Y
中科院分区：
文献类型：
--
作者：
Bae YA;Cai GB;Kim SH;Zo YG;Kong Y
磷脂氢过氧化物谷胱甘肽过氧化物酶 (PHGPx) 是 GPx 家族中最丰富的亚型，直接干扰脂质的氢过氧化。这些蛋白质的生化特性随其供体生物体的不同而变化，这使得多种 PHGPx 类蛋白质的系统发育分类变得复杂。尽管进行了综合分析，但无脊椎动物中 GPx 基因的进化方面仍然很大程度上未知。我们通过计算机筛选真核生物（包括原口物种）的基因组和/或表达序列标签数据库来分离 GPx 同源物。与哺乳动物 PHGPx 基因表现出高度相似性的基因常见于所有检查的基因组中。另外还分别从线虫和扁形动物中检测到 GPx3 和 GPx7 样基因。具有不同生化特性的 PHGPx 样蛋白的总体分布在不同分类群中存在偏差；硒和谷胱甘肽（GSH）依赖性蛋白质仅在扁形动物和后口动物物种中检测到，而硒独立性和硫氧还蛋白（Trx）依赖性酶在其他类群中分离出来。比较基因组组织，GSH 依赖性 PHGPx 基因表现出保守的结构模式，而 Trx 依赖性基因则表现出复杂的外显子-内含子结构。发现参与还原剂结合的解析半胱氨酸的密码子在一系列基因中被取代。在GSH依赖性基因中维持硒代半胱氨酸密码子的选择压力在其进化过程中似乎也被放松了。随着基因组组织的二分方式，其系统发育树的高度多元拓扑意味着 GPx 基因具有多种进化中间形式。无脊椎动物 GPx 基因的比较分析为支持 GPx 进化的模块化途径提供了信息证据，该途径伴随着零星的扩展/缺失和外显子-内含子重塑。差异化的酶性质可能是通过选择压力的进化松弛和/或对作用环境的生化适应而获得的。我们目前的研究将有助于详细了解复杂的 GPx 进化，并了解该抗氧化系统在各自供体生物体中的特殊生理影响的分子基础。
Phospholipid hydroperoxide glutathione peroxidases (PHGPx), the most abundant isoforms of GPx families, interfere directly with hydroperoxidation of lipids. Biochemical properties of these proteins vary along with their donor organisms, which has complicated the phylogenetic classification of diverse PHGPx-like proteins. Despite efforts for comprehensive analyses, the evolutionary aspects of GPx genes in invertebrates remain largely unknown. We isolated GPx homologs via in silico screening of genomic and/or expressed sequence tag databases of eukaryotic organisms including protostomian species. Genes showing strong similarity to the mammalian PHGPx genes were commonly found in all genomes examined. GPx3- and GPx7-like genes were additionally detected from nematodes and platyhelminths, respectively. The overall distribution of the PHGPx-like proteins with different biochemical properties was biased across taxa; selenium- and glutathione (GSH)-dependent proteins were exclusively detected in platyhelminth and deuterostomian species, whereas selenium-independent and thioredoxin (Trx)-dependent enzymes were isolated in the other taxa. In comparison of genomic organization, the GSH-dependent PHGPx genes showed a conserved architectural pattern, while their Trx-dependent counterparts displayed complex exon-intron structures. A codon for the resolving Cys engaged in reductant binding was found to be substituted in a series of genes. Selection pressure to maintain the selenocysteine codon in GSH-dependent genes also appeared to be relaxed during their evolution. With the dichotomized fashion in genomic organizations, a highly polytomic topology of their phylogenetic trees implied that the GPx genes have multiple evolutionary intermediate forms. Comparative analysis of invertebrate GPx genes provides informative evidence to support the modular pathways of GPx evolution, which have been accompanied with sporadic expansion/deletion and exon-intron remodeling. The differentiated enzymatic properties might be acquired by the evolutionary relaxation of selection pressure and/or biochemical adaptation to the acting environments. Our present study would be beneficial to get detailed insights into the complex GPx evolution, and to understand the molecular basis of the specialized physiological implications of this antioxidant system in their respective donor organisms.
登录
查看更多内容
DOI：
10.1093/bioinformatics/8.3.275
发表时间：
1992-06-01
期刊：
COMPUTER APPLICATIONS IN THE BIOSCIENCES
影响因子：
--
作者：
JONES, DT;TAYLOR, WR;THORNTON, JM
通讯作者：
THORNTON, JM
DOI：
10.1111/j.1432-1033.1983.tb07687.x
发表时间：
1983-01-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
GROSSMANN, A;WENDEL, A
通讯作者：
WENDEL, A
影响因子：
56.9
作者：
Kryukov, GV;Castellano, S;Gladyshev, VN
通讯作者：
Gladyshev, VN
影响因子：
12.3
作者：
通讯作者：
--
DOI：
10.1006/bbrc.1996.1525
发表时间：
1996-10-14
影响因子：
3.1
作者：
Arai, M;Imai, H;Nakagawa, Y
通讯作者：
Nakagawa, Y