Isolation and identification of a putative scent-related gene RhMYB1 from rose
Isolation and identification of a putative scent-related gene RhMYB1 from rose
复制标题
玫瑰香味相关基因RhMYB1的分离与鉴定
DOI：
10.1007/s11033-010-0577-1
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发表时间：
2011-10
影响因子：
2.8
通讯作者：
Tang, Kaixue
中科院分区：
文献类型：
--
作者：
Yan, Huijun;Zhang, Hao;Wang, Qigang;Jian, Hongying;Qiu, Xianqin;Wang, Jihua;Tang, Kaixue
玫瑰香味在吸引传粉者和商业价值方面发挥着重要作用。然而，一些与玫瑰花香代谢有关的基因还不太清楚。本文对野生型有香味玫瑰（WR）及其自发无香味突变体玫瑰（MR）进行了分析。SPME-GC/MS分析显示，1-乙基-4-甲氧基苯在WR和mr中所代表的相对含量有显著差异。我们从之前研究的两种玫瑰的SSH文库中分离出一个编码MYB家族转录因子的EST，并命名为RhMYB1。本研究利用cDNA末端快速扩增技术（RACE）对RhMYB1的全长cDNA进行了鉴定和鉴定。RhMYB1全长cDNA为1125 bp，包含882 bp的开放阅读框，编码294个氨基酸的前体蛋白。序列比对表明，RhMYB1与其他植物的r型MYB具有较高的相似性，并且含有一个DNA结合域。Northern blot分析显示，RhMYB1在花瓣中有特异性表达，且在WR中的表达水平随着香味释放量的增加而增加，随着香味释放量的减少而降低。提示RhMYB1可能是一个参与玫瑰香味生物合成的推定鉴定基因。
Rose fragrances play an important role in attracting pollinators and commercial value. However, some genes involved in rose floral scent metabolism are less well understood. Here, wild-type scented rose (WR) and its spontaneous non-scented mutant rose (MR) were analyzed. SPME-GC/MS analysis showed that relative content of 1-ethenyl-4-methoxy-benzene represented was significantly different between WR and MR. We have isolated an EST encoding a MYB family of transcription factor from SSH libraries of the two roses in the previous studies, and designated RhMYB1. In the study, the full-length cDNA of RhMYB1 was identified and characterized by rapid amplification of cDNA ends (RACE). The RhMYB1 full-length cDNA was 1,125 bp containing an 882 bp open reading frame, which encodes a precursor protein of 294 amino acids. Sequence alignments revealed that RhMYB1 shared high similarity with other plants R-type MYB, and RhMYB1 contained a DNA binding domain. Northern blot analysis revealed that RhMYB1 was expressed specifically in flower petal, moreover, the expression level of RhMYB1 in WR increased along with scent emission, and decreased when the scent emission decreased. It is suggested that RhMYB1 might be a putative identification of gene involved in the biosynthesis of rose scent.
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影响因子：
7.4
作者：
Blount, JW;Korth, KL;Dixon, RA
通讯作者：
Dixon, RA
DOI：
10.1021/bk-1993-0525.ch019
发表时间：
1993-04
期刊：
--
影响因子：
--
作者：
I. Flament;C. Debonneville;A. Furrer
通讯作者：
I. Flament;C. Debonneville;A. Furrer
影响因子：
2.8
作者：
Y. Wei;Jia-Na Li;Junxing Lu;Zhanglin Tang;Dong-Chun Pu;Y. Chai
通讯作者：
Y. Wei;Jia-Na Li;Junxing Lu;Zhanglin Tang;Dong-Chun Pu;Y. Chai
DOI：
10.1079/9780851991375.0000
发表时间：
1996-12
期刊：
--
影响因子：
--
作者：
Edward Weiss
通讯作者：
Edward Weiss
DOI：
10.1021/bk-1993-0525
发表时间：
1993-04
期刊：
--
影响因子：
--
作者：
R. Teranishi;R. Buttery;H. Sugisawa
通讯作者：
R. Teranishi;R. Buttery;H. Sugisawa