Nitroprusside ameliorates lung allograft reperfusion injury.
Nitroprusside ameliorates lung allograft reperfusion injury.
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硝普钠可改善肺同种异体移植物再灌注损伤。
DOI：
10.1016/s0003-4975(96)00439-0
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Patterson,GA
中科院分区：
文献类型：
--
作者：
Yamashita,M;Schmid,RA;Ando,K;Cooper,JD;Patterson,GA
背景一氧化氮被认为通过与中性粒细胞、血小板和血管壁细胞成分的相互作用在维持血管完整性方面发挥着关键作用。据报道，内源性一氧化氮水平在缺血、再灌注或两者后被抑制。此外，外源性以及内源性一氧化氮减少再灌注诱导的血管功能障碍。我们假设，硝普钠，一种有效的一氧化氮供体，可能会提高肺保存和减少移植后肺移植dysfunctions.MethodsTen狗进行了左肺同种异体移植。用改良的Euro-Collins溶液冲洗供体肺，并在1 ℃下储存21小时。移植后立即结扎对侧右主肺动脉和支气管，以评估孤立的同种异体移植物功能。处死前6小时评估血流动力学和动脉血气分析（吸入氧分数，1.0）。评估同种异体移植物髓过氧化物酶活性和湿干重比。第1组（n = 5）动物未接受硝普钠。在第2组（n = 5）中，供体肺接受硝普钠冲洗液（10 mg/L），受体动物在再灌注前接受0.2 mg/kg硝普钠，并在评估期间持续输注硝普钠（0.11 ± 0.01 mg · kg-1· h_1）。虽然同种异体移植物髓过氧化物酶的活性和从同种异体移植物抽吸的液体总量显着减少，在组2中，支气管肺泡灌洗液中的蛋白质水平没有统计学差异。ConclusionsNitroprusside管理在冲洗液和再灌注过程中提高肺移植物的功能和血流，并降低肺血管阻力和髓过氧化物酶的活性在移植肺。硝普钠减轻移植肺再灌注损伤。
BackgroundNitric oxide is believed to play a critical role in the maintenance of vascular integrity through its interaction with neutrophils, platelets, and cellular components of the vessel wall. It has been reported that endogenous nitric oxide level was depressed after ischemia, reperfusion, or both. Furthermore, exogenous as well as endogenous nitric oxide decreases reperfusion-induced vascular dysfunction. We hypothesized that nitroprusside, a potent nitric oxide donor, might enhance lung preservation and reduce posttransplantation lung allograft dysfunction.MethodsTen dogs underwent left lung allotransplantation. Donor lungs were flushed with modified Euro-Collins solution and stored for 21 hours at 1°C. Immediately after transplantation, the contralateral right main pulmonary artery and bronchus were ligated to assess isolated allograft function. Hemodynamics and arterial blood gas analysis (inspired oxygen fraction, 1.0) were assessed for 6 hours before sacrifice. Allograft myeloperoxidase activity and wet-to-dry weight ratio were assessed. Group 1 (n = 5) animals received no nitroprusside. In group 2 (n = 5), the donor lung received nitroprusside in the flush solution (10 mg/L) and recipient animals received 0.2 mg/kg just before reperfusion as well as a continuous infusion (0.11 ± 0.01 mg · kg−1· h_1) during the assessment period.ResultsSuperior gas exchange and hemodynamics were noted in lungs receiving nitroprusside. Although allograft myeloperoxidase activity and the total amount of fluid suctioned from the allograft were significantly reduced in group 2, protein levels in bronchoalveolar lavage fluid were not statistically different.ConclusionsNitroprusside administration in the flush solution and during reperfusion improves lung allograft function and blood flow, and reduces pulmonary vascular resistance and myeloperoxidase activity in the transplanted lung. Nitroprusside reduces lung allograft reperfusion injury.
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DOI：
10.1016/s0022-5223(96)70252-0
发表时间：
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期刊：
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ARDS、中性粒细胞和己酮可可碱。
DOI：
--
发表时间：
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期刊：
American Review of Respiratory Disease
影响因子：
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