Potassium secretion by vestibular dark cell epithelium demonstrated by vibrating probe.
Potassium secretion by vestibular dark cell epithelium demonstrated by vibrating probe.
复制标题
振动探针证实前庭暗细胞上皮的钾分泌。
DOI：
10.1016/s0006-3495(94)80987-7
复制
发表时间：
1994
影响因子：
3.4
通讯作者：
Shipley,AM
中科院分区：
文献类型：
--
作者：
Marcus,DC;Shipley,AM
前庭迷路对运动和位置的检测取决于内耳中央室中钾的积累，作为驱动转导过程的能量来源。许多间接证据表明前庭暗细胞 (VDC) 上皮负责在管腔内浓缩 K+。我们使用振动探针技术直接观察该组织产生的电压和离子梯度，从而将这一假设建立在坚实的实验基础上。使用振动电压敏感探针测量 VDC 上皮顶膜上的相对电流密度（Isc，探针），并通过执行已知刺激或抑制跨上皮等效短路电流的操作来验证该技术。基底外侧布美他尼 (5 x 10(-5) M) 和哇巴因 (1 x 10(-3) M) 分别导致 Isc,probe 降低 55 +/- 6% 和 39 +/- 3%，同时将基底外侧 K+ 浓度从 4 mM 提高到 25 mM 导致 Isc,probe 增加 35 +/- 8%。使用振动 K(+) 选择电极检测到指向顶膜的 K+ 梯度，这表明 VDC 上皮确实在控制条件下分泌 K+。这种分泌被布美他尼（94 +/- 7%）和哇巴因（52 +/- 8%）抑制。结果证实了暗细胞产生 K+ 通量的假设，并定性地支持了该通量与跨上皮电流之间的相关性。
Detection of motion and position by the vestibular labyrinth depends on the accumulation of potassium within a central compartment of the inner ear as a source of energy to drive the transduction process. Much circumstantial evidence points to the vestibular dark cell (VDC) epithelium as being responsible for concentrating K+ within the lumen. We have used the vibrating probe technique to directly observe voltage and ion gradients produced by this tissue to put this assumption on a solid experimental footing. Relative current density (Isc,probe) over the apical membrane of VDC epithelium was measured with the vibrating voltage-sensitive probe, and this technique was validated by performing maneuvers known to either stimulate or inhibit the transepithelial equivalent short circuit current. Basolateral bumetanide (5 x 10(-5) M) and ouabain (1 x 10(-3) M) caused a decrease in Isc,probe by 55 +/- 6% and 39 +/- 3%, respectively while raising the basolateral K+ concentration from 4 to 25 mM caused an increase by 35 +/- 8%. A K+ gradient directed toward the apical membrane was detected with the vibrating K(+)-selective electrode, demonstrating that, indeed, the VDC epithelium secretes K+ under control conditions. This secretion was inhibited by bumetanide (by 94 +/- 7%) and ouabain (by 52 +/- 8%). The results substantiate the supposition that dark cells produce a K+ flux and qualitatively support the correlation between this flux and the transepithelial current.
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DOI：
10.1111/1523-1747.ep12526124
发表时间：
1981-04
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Hideo Yaoita;R. A. Briggaman;T. J. Lawley;Thomas T. Provost;Stephen I. Katz
通讯作者：
Hideo Yaoita;R. A. Briggaman;T. J. Lawley;Thomas T. Provost;Stephen I. Katz
皮肤基底膜区。
DOI：
--
发表时间：
1987
期刊：
Advances in dermatology
影响因子：
--
作者：
Fine,JD
通讯作者：
Fine,JD
疱疹样皮炎患者皮肤中 IgA 的免疫电镜定位。
影响因子：
6.5
作者：
H. Yaoita;S. Katz
通讯作者：
S. Katz
影响因子：
10.3
作者：
C. Nieboer;D. Boorsma;M. Woerdeman;G. L. Kalsbeek
通讯作者：
G. L. Kalsbeek
大疱性表皮松解症：一种多种病因的临床疾病。
影响因子：
13.8
作者：
Wilson Bd;Arthur F. Birnkrant;E. H. Beutner;J. Maize
通讯作者：
J. Maize