Lhx2 is required for patterning and expansion of a distinct progenitor cell population committed to eye development.
Lhx2 is required for patterning and expansion of a distinct progenitor cell population committed to eye development.
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DOI：
10.1371/journal.pone.0023387
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Carlsson L
中科院分区：
文献类型：
--
作者：
Hägglund AC;Dahl L;Carlsson L
前体细胞致力于眼睛的发展成为特定的前瞻性前脑和发展成视泡和视杯。视泡诱导透镜基板在表面外胚层中形成，透镜从该基板发育。许多转录因子参与这一过程，包括眼场转录因子。然而，这些转录因子中的许多也调节前神经板的图案化，并且它们在眼睛发育中的具体作用难以辨别，因为眼睛定向祖细胞的定义很差。通过使用Lhx 2启动子的特定部分来调节转基因小鼠中Cre重组酶的表达，我们已经能够在前脑中定义一个单独致力于眼睛发育的独特祖细胞群体。这些祖细胞中Lhx 2的条件性失活导致眼发育在视泡诱导表面外胚层中透镜基板形成的阶段停滞。眼定向祖细胞群存在于Lhx 2 −/−胚胎前脑中，表明眼发育的定向是Lhx 2独立的。然而，Lhx 2在Lhx 2 −/−祖细胞中的重新表达仅促进视网膜色素上皮细胞的发育，表明Lhx 2促进这些祖细胞的寡能命运的获得。这种方法还使我们能够识别出Lhx 2在眼睛发育中的功能与前脑中的功能不同的基因。因此，我们已经确定了一个独特的祖细胞群体在前脑致力于眼睛的发展，并确定了基因与Lhx 2的功能，在这些祖细胞的扩展和模式。
Progenitor cells committed to eye development become specified in the prospective forebrain and develop subsequently into the optic vesicle and the optic cup. The optic vesicle induces formation of the lens placode in surface ectoderm from which the lens develops. Numerous transcription factors are involved in this process, including the eye-field transcription factors. However, many of these transcription factors also regulate the patterning of the anterior neural plate and their specific role in eye development is difficult to discern since eye-committed progenitor cells are poorly defined. By using a specific part of the Lhx2 promoter to regulate Cre recombinase expression in transgenic mice we have been able to define a distinct progenitor cell population in the forebrain solely committed to eye development. Conditional inactivation of Lhx2 in these progenitor cells causes an arrest in eye development at the stage when the optic vesicle induces lens placode formation in the surface ectoderm. The eye-committed progenitor cell population is present in the Lhx2−/− embryonic forebrain suggesting that commitment to eye development is Lhx2-independent. However, re-expression of Lhx2 in Lhx2−/− progenitor cells only promotes development of retinal pigment epithelium cells, indicating that Lhx2 promotes the acquisition of the oligopotent fate of these progenitor cells. This approach also allowed us to identify genes that distinguish Lhx2 function in eye development from that in the forebrain. Thus, we have defined a distinct progenitor cell population in the forebrain committed to eye development and identified genes linked to Lhx2's function in the expansion and patterning of these progenitor cells.
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影响因子：
16.2
作者：
Cavodeassi F;Carreira-Barbosa F;Young RM;Concha ML;Allende ML;Houart C;Tada M;Wilson SW
通讯作者：
Wilson SW
影响因子：
56.9
作者：
Mangale, Vishakha S.;Hirokawa, Karla E.;Satyaki, Prasad R. V.;Gokulchandran, Nandini;Chikbire, Satyadeep;Subramanian, Lakshmi;Shetty, Ashwin S.;Martynoga, Ben;Paul, Jolly;Mai, Mark V.;Li, Yuqing;Flanagan, Lisa A.;Tole, Shubha;Monuki, Edwin S.
通讯作者：
Monuki, Edwin S.
影响因子：
4.6
作者：
Lavado, Alfonso;Lagutin, Oleg V.;Oliver, Guillermo
通讯作者：
Oliver, Guillermo
影响因子：
10.5
作者：
Ashery-Padan, R;Marquardt, T;Gruss, P
通讯作者：
Gruss, P
影响因子：
10.5
作者：
DUDLEY, AT;LYONS, KM;ROBERTSON, EJ
通讯作者：
ROBERTSON, EJ