Less Is More: Oligomer Extraction and Hydrothermal Annealing Increase PDMS Adhesion Forces for Materials Studies and for Biology-Focused Microfluidic Applications.
Less Is More: Oligomer Extraction and Hydrothermal Annealing Increase PDMS Adhesion Forces for Materials Studies and for Biology-Focused Microfluidic Applications.
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DOI：
10.3390/mi14010214
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发表时间：
2023-01-14
期刊：
影响因子：
3.4
通讯作者：
Gillette, Martha U.
中科院分区：
文献类型：
--
作者：
Millet, Larry J.;Jain, Anika;Gillette, Martha U.
关键词：
微环境中的线索是复杂细胞形态和功能出现的关键决定因素。其中最主要的是形成网络的相邻小区的存在。对于高分辨率分析，开发允许精确控制网络形成的微环境至关重要。在细胞科学、组织工程和临床生物学中尤其如此。我们引入了一种组装基于聚二甲基硅氧烷（PDMS）的微流体环境的新方法，可增强细胞网络的形成和分析。我们报告说，PDMS 溶剂萃取和水热退火的组合过程创造了独特的条件，可以在溶剂萃取的 PDMS (E-PDMS) 和玻璃之间产生高强度的键合，而这些特性与传统 PDMS 不相关。萃取后进行水热退火可去除未结合的低聚物，促进聚合物交联，促进与玻璃形成共价键，并保留最高的生物相容性。在此，我们的提取方案可将低聚物的去除速度从 5 天缩短至 2 天。由此产生的微流体平台由于高粘附力而特别适合细胞网络研究，有效地阻止细胞延伸并消除有害的低聚物。我们展示了多个微流体域的简单、同时驱动，用于在神经胶质细胞网络中调用 ATP 和谷氨酸诱导的 Ca2+ 信号传导。这些 E-PDMS 修改和流动操作进一步使微流体技术能够用于细胞信号传导和网络研究以及新颖的应用。
Cues in the micro-environment are key determinants in the emergence of complex cellular morphologies and functions. Primary among these is the presence of neighboring cells that form networks. For high-resolution analysis, it is crucial to develop micro-environments that permit exquisite control of network formation. This is especially true in cell science, tissue engineering, and clinical biology. We introduce a new approach for assembling polydimethylsiloxane (PDMS)-based microfluidic environments that enhances cell network formation and analyses. We report that the combined processes of PDMS solvent-extraction and hydrothermal annealing create unique conditions that produce high-strength bonds between solvent-extracted PDMS (E-PDMS) and glass—properties not associated with conventional PDMS. Extraction followed by hydrothermal annealing removes unbound oligomers, promotes polymer cross-linking, facilitates covalent bond formation with glass, and retains the highest biocompatibility. Herein, our extraction protocol accelerates oligomer removal from 5 to 2 days. Resulting microfluidic platforms are uniquely suited for cell-network studies owing to high adhesion forces, effectively corralling cellular extensions and eliminating harmful oligomers. We demonstrate the simple, simultaneous actuation of multiple microfluidic domains for invoking ATP- and glutamate-induced Ca2+ signaling in glial-cell networks. These E-PDMS modifications and flow manipulations further enable microfluidic technologies for cell-signaling and network studies as well as novel applications.
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DOI：
10.1523/jneurosci.0203-12.2012
发表时间：
2012-05-30
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Hosmane S;Tegenge MA;Rajbhandari L;Uapinyoying P;Ganesh Kumar N;Thakor N;Venkatesan A
通讯作者：
Venkatesan A
影响因子：
6.1
作者：
Hosmane, Suneil;Yang, In Hong;Venkatesan, Arun
通讯作者：
Venkatesan, Arun
影响因子：
6.1
作者：
Jo, Kyubong;Heien, Michael L.;Sweedler, Jonathan V.
通讯作者：
Sweedler, Jonathan V.
影响因子：
13.3
作者：
Brimmo, Ayoola T.;Menachery, Anoop;Qasaimeh, Mohammad A.
通讯作者：
Qasaimeh, Mohammad A.
影响因子：
2.2
作者：
Fritz, JL;Owen, MJ
通讯作者：
Owen, MJ