A comprehensive overview of grain development in Brachypodium distachyon variety Bd21.
A comprehensive overview of grain development in Brachypodium distachyon variety Bd21.
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DOI：
10.1093/jxb/err298
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发表时间：
2012-01
影响因子：
6.9
通讯作者：
Dubreucq B
中科院分区：
文献类型：
--
作者：
Guillon F;Larré C;Petipas F;Berger A;Moussawi J;Rogniaux H;Santoni A;Saulnier L;Jamme F;Miquel M;Lepiniec L;Dubreucq B
详细而全面地了解种子储备积累对农业和作物改良策略具有重要意义。这项工作是一项研究计划的一部分，旨在使用Brachypodium distachyon作为模式植物的谷物颗粒的发展和填充。重点是Bd 21 -3加入，收集形态，细胞学和生化数据，包括蛋白质，脂质，糖，淀粉和细胞壁分析在粮食发展。本研究强调了三个主要发育阶段的存在，在Brachypodium颖果，并提供了一个广泛的描述Brachypodium粮食发展。在第一阶段，即形态发生，胚胎迅速发育，在受精后约18 d（18 d）达到其最终形态。在同一时期，胚乳增大，最终占籽粒体积的80%。在成熟阶段，碳水化合物不断储存，主要在胚乳中，从蔗糖转换为淀粉积累。大量的β-葡聚糖积累在胚乳中，沉积模式的局部变化。有趣的是，与其他谷物相比，在短柄草中发现了新的β-葡聚糖。从1500年起，发现大量蛋白质（即球蛋白和醇溶谷蛋白）。这些蛋白质储存在两种不同的亚细胞结构中，这两种结构也存在于水稻中，但在普安亚科中并不常见。在发育后期，籽粒脱水，而干物质保持相当稳定。短柄草属植物与驯化的谷类植物有一些显著的差异。β-葡聚糖在谷粒发育过程中积累，并且这种细胞壁多糖是以淀粉为代价的主要储存碳水化合物。
A detailed and comprehensive understanding of seed reserve accumulation is of great importance for agriculture and crop improvement strategies. This work is part of a research programme aimed at using Brachypodium distachyon as a model plant for cereal grain development and filling. The focus was on the Bd21-3 accession, gathering morphological, cytological, and biochemical data, including protein, lipid, sugars, starch, and cell-wall analyses during grain development. This study highlighted the existence of three main developmental phases in Brachypodium caryopsis and provided an extensive description of Brachypodium grain development. In the first phase, namely morphogenesis, the embryo developed rapidly reaching its final morphology about 18 d after fertilization (DAF). Over the same period the endosperm enlarged, finally to occupy 80% of the grain volume. During the maturation phase, carbohydrates were continuously stored, mainly in the endosperm, switching from sucrose to starch accumulation. Large quantities of β-glucans accumulated in the endosperm with local variations in the deposition pattern. Interestingly, new β-glucans were found in Brachypodium compared with other cereals. Proteins (i.e. globulins and prolamins) were found in large quantities from 15 DAF onwards. These proteins were stored in two different sub-cellular structures which are also found in rice, but are unusual for the Pooideae. During the late stage of development, the grain desiccated while the dry matter remained fairly constant. Brachypodium exhibits some significant differences with domesticated cereals. Beta-glucan accumulates during grain development and this cell wall polysaccharide is the main storage carbohydrate at the expense of starch.
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影响因子：
3.7
作者：
Febrer M;Goicoechea JL;Wright J;McKenzie N;Song X;Lin J;Collura K;Wissotski M;Yu Y;Ammiraju JS;Wolny E;Idziak D;Betekhtin A;Kudrna D;Hasterok R;Wing RA;Bevan MW
通讯作者：
Bevan MW
影响因子：
6.9
作者：
Guillon, Fabienne;Bouchet, Brigitte;Saulnier, Luc
通讯作者：
Saulnier, Luc
影响因子：
4.4
作者：
Devouge, Vanessa;Rogniaux, Helene;Larre, Colette
通讯作者：
Larre, Colette
影响因子：
6.5
作者：
Buckeridge, MS;dos Santos, HP;Tiné, MAS
通讯作者：
Tiné, MAS
影响因子：
7.2
作者：
Borisjuk, L;Walenta, S;Weber, H
通讯作者：
Weber, H