DNA-Directed Patterning for Versatile Validation and Characterization of a Lipid-Based Nanoparticle Model of SARS-CoV-2.
DNA-Directed Patterning for Versatile Validation and Characterization of a Lipid-Based Nanoparticle Model of SARS-CoV-2.
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DNA 定向图案化，用于 SARS-CoV-2 脂质纳米颗粒模型的多功能验证和表征。
DOI：
10.1002/advs.202101166
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发表时间：
2021-12
期刊：
影响因子：
--
通讯作者：
Sohn LL
中科院分区：
文献类型：
--
作者：
Kozminsky M;Carey TR;Sohn LL
基于脂质的纳米颗粒已广泛应用于药物递送和疫苗策略，并在2019冠状病毒病（COVID-19）大流行中发现了多种应用-从疫苗组分封装到病毒本身建模。高通量、高度灵活的表征方法对开发具有表面蛋白的脂质体非常有益。DNA定向图案化是一种这样的方法，其在固定和分离基于脂质的纳米颗粒以用于后续分析方面提供了多功能性。在这里，寡核苷酸被选择性地缀合到玻璃基底上，然后与标记到脂质体的互补寡核苷酸杂交，以极大的控制和精确度对其进行图案化。通过表征严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的新型重演性脂质基纳米颗粒模型-S-脂质体-证明了DNA定向图案化的力量，该模型在其表面上呈现SARS-CoV-2刺突（S）蛋白。将S-脂质体和将四跨膜蛋白CD 63展示于底物的离散区域的脂质体的混合物图案化，表明血管紧张素转换酶2（ACE 2）特异性结合S-脂质体。随后将S-脂质体引入表达ACE 2的细胞中检测了S-脂质体的生物学功能，并显示与基于DNA定向图案化的测定一致。最后，S-脂质体的多重图案化验证了针对两种S变体的市售中和抗体的性能。总的来说，DNA定向图案化能够实现各种各样的定制测定，用于表征任何基于脂质的纳米颗粒。DNA指导的脂质体图案化是一种对不同固定化纳米颗粒群体进行高通量表征的新方法。通过测定血管紧张素转换酶2的结合以及广泛中和抗体的有效性，验证了严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的脂质纳米颗粒模型，即加标脂质体。
Lipid‐based nanoparticles have been applied extensively in drug delivery and vaccine strategies and are finding diverse applications in the coronavirus disease 2019 (COVID‐19) pandemic—from vaccine‐component encapsulation to modeling the virus, itself. High‐throughput, highly flexible methods for characterization are of great benefit to the development of liposomes featuring surface proteins. DNA‐directed patterning is one such method that offers versatility in immobilizing and segregating lipid‐based nanoparticles for subsequent analysis. Here, oligonucleotides are selectively conjugated onto a glass substrate and then hybridized to complementary oligonucleotides tagged to liposomes, patterning them with great control and precision. The power of DNA‐directed patterning is demonstrated by characterizing a novel recapitulative lipid‐based nanoparticle model of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2)—S–liposomes—that presents the SARS‐CoV‐2 spike (S) protein on its surface. Patterning a mixture of S–liposomes and liposomes that display the tetraspanin CD63 to discrete regions of a substrate shows that angiotensin‐converting enzyme 2 (ACE2) specifically binds to S–liposomes. Subsequent introduction of S–liposomes to ACE2‐expressing cells tests the biological function of S–liposomes and shows agreement with DNA‐directed patterning‐based assays. Finally, multiplexed patterning of S–liposomes verifies the performance of commercially available neutralizing antibodies against the two S variants. Overall, DNA‐directed patterning enables a wide variety of custom assays for the characterization of any lipid‐based nanoparticle. DNA‐directed patterning of liposomes is a novel method to perform high‐throughput characterization of different populations of immobilized nanoparticles. It is demonstrated by validating a lipid‐based nanoparticle model of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2), spike–liposomes, through assays that measure binding of angiotensin‐converting enzyme 2 as well as the efficacy of broadly neutralizing antibodies.
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DOI：
10.1056/nejmoa2029849
发表时间：
2021-01-21
期刊：
The New England journal of medicine
影响因子：
--
作者：
Chen P;Nirula A;Heller B;Gottlieb RL;Boscia J;Morris J;Huhn G;Cardona J;Mocherla B;Stosor V;Shawa I;Adams AC;Van Naarden J;Custer KL;Shen L;Durante M;Oakley G;Schade AE;Sabo J;Patel DR;Klekotka P;Skovronsky DM;BLAZE-1 Investigators
通讯作者：
BLAZE-1 Investigators
DOI：
10.1073/pnas.1521230113
发表时间：
2016-02-23
影响因子：
11.1
作者：
Kowal, Joanna;Arras, Guillaume;Thery, Clotilde
通讯作者：
Thery, Clotilde
影响因子：
15
作者：
Lee, Jiseok;Kim, Hyong-Jun;Kim, Jinsang
通讯作者：
Kim, Jinsang
影响因子：
64.8
作者：
MARTIN, F;MACDONAL.R
通讯作者：
MACDONAL.R
影响因子：
64.8
作者：
Barnes CO;Jette CA;Abernathy ME;Dam KA;Esswein SR;Gristick HB;Malyutin AG;Sharaf NG;Huey-Tubman KE;Lee YE;Robbiani DF;Nussenzweig MC;West AP Jr;Bjorkman PJ
通讯作者：
Bjorkman PJ