Superficially porous silica particles with wide pores for biomacromolecular separations.
Superficially porous silica particles with wide pores for biomacromolecular separations.
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DOI：
10.1016/j.chroma.2012.09.052
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发表时间：
2012-11-16
期刊：
影响因子：
--
通讯作者：
Kirkland JJ
中科院分区：
文献类型：
--
作者：
Wagner BM;Schuster SA;Boyes BE;Kirkland JJ
自2006年以来，表面多孔颗粒(SPP)柱(通常称为熔核、多孔壳或核-壳颗粒)对高效液相色谱分离产生了严重影响。这些颗粒的孔径约为100？，专为分离小分子而设计。最近，具有160-200ä孔径的SPP已被用于分离多肽和小蛋白。这份报告描述了融合核心颗粒大小、孔径大小、壳层厚度和配体类型对快速、有效地分离较大分子(如完整蛋白质和其他至少400 kDa的生物大分子)的影响。这些参数的优化导致了颗粒没有显示出会影响大生物分子分离效率的限制扩散。薄的多孔壳为这些大分子提供了出色的传质(动力学)，与传统的全多孔颗粒相比，导致了更好的分离。可以调整样品载量，以便对复杂混合物中的次要成分具有良好的检测灵敏度。强大的颗粒强度保证了稳定、高效的柱子加载。对含有不同烷基配体的固定相进行了测试，以提供关于蛋白质的保留、柱效和峰形的数据。这些新的宽孔熔融核心颗粒的开发现在可以利用SPP配置的优势，实现对各种不同大小分子的高效液相分离。
Since 2006, columns of superficially porous particles (SPP), often called Fused-core®, porous-shell or core-shell particles, have had serious impact on HPLC separations. These particles have pore diameters of about 100 Å designed for separating small molecules. More recently, SPP with 160 - 200 Å pore diameter have been made available for separating peptides and small proteins. This report describes the effects of fused-core particle size, pore size, shell thickness and ligand type for the rapid, efficient separation of larger molecules such as intact proteins and other biomacromolecules up to at least 400 kDa. Optimization of these parameters resulted in particles that show no restricted diffusion that would compromise separating efficiency for large biomolecules. The thin porous shell provides excellent mass transfer (kinetics) for these large molecules, resulting in superior separations compared to conventional totally porous particles. Sample loading capacity can be adjusted to allow good detection sensitivity for minor components in a complex mixture. Strong particle strength ensures the loading of stable, high-efficiency columns. Stationary phases with different alkyl ligands were tested to provide data on retention, column efficiency and peak shapes for proteins. The development of these new wide-pore fused-core particles now allows the HPLC separation of a wide range of molecules of different sizes with advantages of the SPP configuration.
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影响因子：
3.1
作者：
Baker, Jared S.;Vinci, John C.;Colon, Luis A.
通讯作者：
Colon, Luis A.
影响因子：
7.4
作者：
KIRKLAND, JJ
通讯作者：
KIRKLAND, JJ
影响因子：
7.4
作者：
KIRKLAND, JJ;GLAJCH, JL;FARLEE, RD
通讯作者：
FARLEE, RD
DOI：
10.1016/j.jpba.2009.05.011
发表时间：
2009-10-15
影响因子：
3.4
作者：
Song, Wei;Pabbisetty, Deepthi;Fast, Douglas M.
通讯作者：
Fast, Douglas M.
影响因子：
7.4
作者：
Liu, XD;Zhou, DM;Guiochon, G
通讯作者：
Guiochon, G