Image-predicated sorting of adherent cells using photopatterned hydrogels.
Image-predicated sorting of adherent cells using photopatterned hydrogels.
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DOI：
10.1002/adhm.201200196
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发表时间：
2013-04
影响因子：
10
通讯作者：
Voldman, Joel
中科院分区：
文献类型：
--
作者：
Kovac, Joseph;Gerardin, Ylaine;Voldman, Joel
Images of cells convey a wealth of information. Researchers routinely use ubiquitous light microscopes to obtain information about cell structure,[1] shape and motility,[2] cell-cell interactions,[3] and protein expression with spatio-temporal resolution.[4] Cell fate prediction using image-based phenotyping is applied to a wide range of cell types, including stem cells.[5, 6] The ability to sort cells following such imaging could offer a number of advantages, such as allowing the direct utilization of cells prospectively identified by imaged phenotypes,[5] and allowing investigation into heterogeneity [7] observed via imaging by applying bulk assays to specific cell subpopulations. When developing cell lines, the most visually promising post-transformation clones could be enriched prior to the protracted efforts of dilution cloning. Pooled, barcoded genetic screens, typically limited to phenotypes that can be sorted via fluorescence activated cell sorting (FACS)[8] or that alter proliferation,[9] could be expanded to all phenotypes recognizable through microscopy. Unfortunately, facile methods to isolate cells based upon that rich, image-derived information are lacking. FACS offers high throughput,[10] but as it does not image cells, it lacks sub-cellular and single-cell temporal resolution, and loses most morphological information because cells are analyzed in suspension. Sorting can be added to microscopy, via live-cell adaptations of laser capture microdissection,[11] laser-based killing of undesired cells,[12] or clone picking following imaging. However, these platforms are all prohibitively expensive for individual labs and/or have specific limitations (proprietary culture films,[11] semi-solid media), and thus have not been widely adopted. Here we present a user-friendly, inexpensive method utilizing photopatternable hydrogels to sort cells following imaging. Termed Polymerization-Activated Cell Sorting (PACS), the method utilizes commercially available reagents and hardware found in most biology labs to create an in-lab
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影响因子：
1.2
作者：
Guryanova, O. A.;Makhanov, M.;Frolova, E. I.
通讯作者：
Frolova, E. I.
DOI：
10.1073/pnas.94.3.849
发表时间：
1997-02-04
影响因子：
11.1
作者：
Maniotis, AJ;Chen, CS;Ingber, DE
通讯作者：
Ingber, DE
影响因子：
64.8
作者：
Keren, Kinneret;Pincus, Zachary;Theriot, Julie A.
通讯作者：
Theriot, Julie A.
影响因子：
4.9
作者：
Khademhosseini, Ali;Eng, George;Burdick, Jason A.
通讯作者：
Burdick, Jason A.
影响因子：
14
作者：
Pagoria, D;Lee, A;Geurtsen, W
通讯作者：
Geurtsen, W