The evolutionally-conserved function of group B1 Sox family members confers the unique role of Sox2 in mouse ES cells.
The evolutionally-conserved function of group B1 Sox family members confers the unique role of Sox2 in mouse ES cells.
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DOI：
10.1186/s12862-016-0755-4
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发表时间：
2016-08-31
影响因子：
3.4
通讯作者：
Ohtsuka S
中科院分区：
文献类型：
--
作者：
Niwa H;Nakamura A;Urata M;Shirae-Kurabayashi M;Kuraku S;Russell S;Ohtsuka S
在小鼠ES细胞中，Sox2的功能对于维持多能性是必不可少的。由于SOX家族转录因子在动物界中非常保守，从理解多能性起源的角度来解决SOX2功能在多能干细胞中的进化起源是很有趣的。在这里，我们通过在可诱导的Sox2缺失的ES细胞中进行功能互补分析来解决这个问题。对不同组别的小鼠SOX蛋白进行检测，我们发现只有B1组和G组蛋白能够支持多能性。有趣的是，哺乳动物B1组Sox蛋白的无脊椎动物同源物能够取代小鼠Sox2的多能性相关功能。此外，被果蝇SoxNeuro蛋白拯救的小鼠ES细胞能够促进嵌合胚胎的形成。这些数据表明，小鼠Sox2支持多能性的功能是基于B1Sox家族在进化上保守的活动。由于发育过程中的多能干细胞群体可以被视为脊椎动物的进化新奇事物，它可以被认为是对其进化保守功能的共同选择。本文的在线版本(doi：10.1186/s12862-0160755-4)包含补充材料，授权用户可以使用。
In mouse ES cells, the function of Sox2 is essential for the maintenance of pluripotency. Since the Sox-family of transcription factors are well conserved in the animal kingdom, addressing the evolutionary origin of Sox2 function in pluripotent stem cells is intriguing from the perspective of understanding the origin of pluripotency. Here we approach this question using a functional complementation assay in inducible Sox2-null ES cells. Assaying mouse Sox proteins from different Groups, we found that only Group B1 and Group G proteins were able to support pluripotency. Interestingly, invertebrate homologs of mammalian Group B1 Sox proteins were able to replace the pluripotency-associated function of mouse Sox2. Moreover, the mouse ES cells rescued by the Drosophila SoxNeuro protein are able to contribute to chimeric embryos. These data indicate that the function of mouse Sox2 supporting pluripotency is based on an evolutionally conserved activity of the Group B1 Sox family. Since pluripotent stem cell population in developmental process could be regarded as the evolutional novelty in vertebrates, it could be regarded as a co-optional use of their evolutionally conserved function. The online version of this article (doi:10.1186/s12862-016-0755-4) contains supplementary material, which is available to authorized users.
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影响因子：
23.9
作者：
Smith KN;Singh AM;Dalton S
通讯作者：
Dalton S
Sox7对于与小鼠ES细胞的原始内胚层分化是可分配的。
影响因子：
--
作者：
Kinoshita M;Shimosato D;Yamane M;Niwa H
通讯作者：
Niwa H
影响因子：
4.6
作者：
Guo, Ge;Yang, Jian;Smith, Austin
通讯作者：
Smith, Austin
影响因子：
21.3
作者：
Jiang, Jianming;Chan, Yun-Shen;Ng, Huck-Hui
通讯作者：
Ng, Huck-Hui
影响因子：
10.7
作者：
Hemmrich, Georg;Khalturin, Konstantin;Bosch, Thomas C. G.
通讯作者：
Bosch, Thomas C. G.