Remote calorimetric detection of urea via flow injection analysis.
Remote calorimetric detection of urea via flow injection analysis.
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通过流动注射分析对尿素进行远程量热检测。
DOI：
10.1039/c5an01306b
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Tadigadapa,Srinivas
中科院分区：
文献类型：
--
作者：
Gaddes,DavidE;Demirel,MelikC;Reeves,WBrian;Tadigadapa,Srinivas
报道了一种能够实现相对高通量样品分析的量热生物传感系统的设计和开发。量热生物传感器系统由一个薄的(∼20μm)微机械Y形石英晶体谐振器作为温度传感器组成，放置在一个装有固定化酶的流体室附近。对尿素酶的层层固定化进行了研究，并对其活性与层数、pH和时间的关系进行了评价。这种构造实现了传感系统，其中换能器元件与感兴趣的分析物溶液在物理上分开，从而不受通常与传感器表面上发生的生化反应相关的污垢影响。通过流动注射分析(FIA)技术检测磷酸盐缓冲液中1-200 mm的尿素，验证了该生物传感系统的性能。小型化的流体系统被用来提供流过反应塔的连续流动。在此构型下，该生物传感器的极限分辨率小于1 mm尿素，在0-50 mm范围内呈现线性响应。这项工作证明了一种传感器本身不会被感兴趣的溶液污染或污染的传感方式，酶固定化Kapton®流体反应柱可以用作一次性色谱柱。这样的系统可实现长期采样测量的重复使用和可靠性。基于这一概念，设想了一种生物传感系统，该系统可以进行快速测量，以在较长时间内连续检测尿液和血液中的葡萄糖、肌酐、胆固醇、尿素和乳酸等生物标志物。
The design and development of a calorimetric biosensing system enabling relatively high throughput sample analysis are reported. The calorimetric biosensor system consists of a thin (∼20 μm) micromachined Y-cut quartz crystal resonator (QCR) as a temperature sensor placed in close proximity to a fluidic chamber packed with an immobilized enzyme. Layer by layer enzyme immobilization of urease is demonstrated and its activity as a function of the number of layers, pH, and time has been evaluated. This configuration enables a sensing system where a transducer element is physically separated from the analyte solution of interest and is thereby free from fouling effects typically associated with biochemical reactions occuring on the sensor surface. The performance of this biosensing system is demonstrated by detection of 1–200 mM urea in phosphate buffer via a flow injection analysis (FIA) technique. Miniaturized fluidic systems were used to provide continuous flow through a reaction column. Under this configuration the biosensor has an ultimate resolution of less than 1 mM urea and showed a linear response between 0–50 mM. This work demonstrates a sensing modality in which the sensor itself is not fouled or contaminated by the solution of interest and the enzyme immobilized Kapton® fluidic reaction column can be used as a disposable cartridge. Such a system enables reuse and reliability for long term sampling measurements. Based on this concept a biosensing system is envisioned which can perform rapid measurements to detect biomarkers such as glucose, creatinine, cholesterol, urea and lactate in urine and blood continuously over extended periods of time.
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影响因子：
12.6
作者：
Tyagi, Manisha;Tomar, Monika;Gupta, Vinay
通讯作者：
Gupta, Vinay
pH 静态酶传感器的传感器特性评估
DOI：
--
发表时间：
1990
期刊：
影响因子：
--
作者：
B. V. Schoot;P. Bergveld
通讯作者：
P. Bergveld
pH 和尿素传感器的光谱研究和优化
DOI：
--
发表时间：
1992
期刊：
影响因子：
--
作者：
G. Gauglitz;M. Reichert
通讯作者：
M. Reichert
借助酶热敏电阻对糖和糖衍生物进行量热分析。
影响因子：
3.1
作者：
Bo Mattiasson;Bengt Danielsson
通讯作者：
Bengt Danielsson